TY - JOUR
T1 - Metabolic Profiling for Evaluating the Dipeptidyl Peptidase-IV Inhibitory Potency of Diverse Green Tea Cultivars and Determining Bioactivity-Related Ingredients and Combinations
AU - Fujimura, Yoshinori
AU - Watanabe, Mototsugu
AU - Morikawa-Ichinose, Tomomi
AU - Fujino, Konatsu
AU - Yamamoto, Mao
AU - Nishioka, Seita
AU - Inoue, Chihiro
AU - Ogawa, Fumiyo
AU - Yonekura, Madoka
AU - Nakasone, Akari
AU - Kumazoe, Motofumi
AU - Tachibana, Hirofumi
N1 - Funding Information:
This work was supported in part by the JSPS KAKENHI grant JP20H05683 awarded to H.T., JP20H02935 to Y.F., and JP20K05960 to M.K.
Publisher Copyright:
© 2022 American Chemical Society. All rights reserved.
PY - 2022/6/1
Y1 - 2022/6/1
N2 - There are numerous cultivars of tea (Camellia sinensis L.), but the differences in their anti-hyperglycemic-related effects are largely unknown. The inhibition of the dipeptidyl peptidase (DPP)-IV enzyme plays an essential role in controlling hyperglycemia in diabetes by blocking the degradation of incretin hormones, which is necessary for insulin secretion. In this study, we examined the DPP-IV inhibitory activity of leaf extracts from diverse Japanese green tea cultivars. The inhibitory rates differed among tea extracts. Metabolic profiling (MP), using liquid chromatography-mass spectrometry, of all cultivars revealed compositional differences among cultivars according to their DPP-IV inhibitory capacity. Epigallocatechin-3-O-(3-O-methyl)gallate, kaempferol-3-O-rutinoside, myricetin-3-O-glucoside/galactoside, and theogallin were newly identified as DPP-IV inhibitors. The bioactivity of a tea extract was potentiated by adding these ingredients in combination. Our results show that MP is a useful approach for evaluating the DPP-IV inhibitory potency of green tea and for determining bioactivity-related ingredients and combinations.
AB - There are numerous cultivars of tea (Camellia sinensis L.), but the differences in their anti-hyperglycemic-related effects are largely unknown. The inhibition of the dipeptidyl peptidase (DPP)-IV enzyme plays an essential role in controlling hyperglycemia in diabetes by blocking the degradation of incretin hormones, which is necessary for insulin secretion. In this study, we examined the DPP-IV inhibitory activity of leaf extracts from diverse Japanese green tea cultivars. The inhibitory rates differed among tea extracts. Metabolic profiling (MP), using liquid chromatography-mass spectrometry, of all cultivars revealed compositional differences among cultivars according to their DPP-IV inhibitory capacity. Epigallocatechin-3-O-(3-O-methyl)gallate, kaempferol-3-O-rutinoside, myricetin-3-O-glucoside/galactoside, and theogallin were newly identified as DPP-IV inhibitors. The bioactivity of a tea extract was potentiated by adding these ingredients in combination. Our results show that MP is a useful approach for evaluating the DPP-IV inhibitory potency of green tea and for determining bioactivity-related ingredients and combinations.
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U2 - 10.1021/acs.jafc.2c01693
DO - 10.1021/acs.jafc.2c01693
M3 - Article
C2 - 35543229
AN - SCOPUS:85130760783
SN - 0021-8561
VL - 70
SP - 6455
EP - 6466
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 21
ER -
