Abstract
Owing to its favorable properties, such as low viscosity and high diffusivity, a supercritical fluid can be used as the mobile phase in chromatography. Supercritical fluid chromatography (SFC) can provide high-speed and high-resolution separation. Since supercritical carbon dioxide (SCCO 2), which is generally used as the mobile phase in SFC, is automatically emitted at room temperature, SFC is most commonly used as a preparative method. However, SFC can also be used to perform high-precision biomolecular analysis, especially for hydrophobic metabolites, because of the low polarity of SCCO2. The use of a mass spectrometer with SFC can widen the scope of application of SFC to bioanalysis. In this review, we summarize practical application of SFC as a tool for the analysis of metabolites in real biological samples.
| Original language | English |
|---|---|
| Pages (from-to) | 27-34 |
| Number of pages | 8 |
| Journal | Bioanalysis |
| Volume | 2 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 2010 |
| Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Pharmacology, Toxicology and Pharmaceutics(all)
- Clinical Biochemistry
- Medical Laboratory Technology