Abstract
Owing to its favorable properties, such as low viscosity and high diffusivity, a supercritical fluid can be used as the mobile phase in chromatography. Supercritical fluid chromatography (SFC) can provide high-speed and high-resolution separation. Since supercritical carbon dioxide (SCCO 2), which is generally used as the mobile phase in SFC, is automatically emitted at room temperature, SFC is most commonly used as a preparative method. However, SFC can also be used to perform high-precision biomolecular analysis, especially for hydrophobic metabolites, because of the low polarity of SCCO2. The use of a mass spectrometer with SFC can widen the scope of application of SFC to bioanalysis. In this review, we summarize practical application of SFC as a tool for the analysis of metabolites in real biological samples.
Original language | English |
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Pages (from-to) | 27-34 |
Number of pages | 8 |
Journal | Bioanalysis |
Volume | 2 |
Issue number | 1 |
DOIs | |
Publication status | Published - Jan 1 2010 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Analytical Chemistry
- Pharmacology, Toxicology and Pharmaceutics(all)
- Clinical Biochemistry
- Medical Laboratory Technology