Metabolome analysis of Drosophila melanogaster during embryogenesis

Phan Nguyen Thuy An, Masamitsu Yamaguchi, Takeshi Bamba, Eiichiro Fukusaki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The Drosophila melanogaster embryo has been widely utilized as a model for genetics and developmental biology due to its small size, short generation time, and large brood size. Information on embryonic metabolism during developmental progression is important for further understanding the mechanisms of Drosophila embryogenesis. Therefore, the aim of this study is to assess the changes in embryos' metabolome that occur at different stages of the Drosophila embryonic development. Time course samples of Drosophila embryos were subjected to GC/MS-based metabolome analysis for profiling of low molecular weight hydrophilic metabolites, including sugars, amino acids, and organic acids. The results showed that the metabolic profiles of Drosophila embryo varied during the course of development and there was a strong correlation between the metabolome and different embryonic stages. Using the metabolome information, we were able to establish a prediction model for developmental stages of embryos starting from their high-resolution quantitative metabolite composition. Among the important metabolites revealed from our model, we suggest that different amino acids appear to play distinct roles in different developmental stages and an appropriate balance in trehalose-glucose ratio is crucial to supply the carbohydrate source for the development of Drosophila embryo.

Original languageEnglish
Article numbere99519
JournalPloS one
Volume9
Issue number8
DOIs
Publication statusPublished - Aug 14 2014

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Metabolome
metabolomics
Metabolites
Drosophila melanogaster
embryo (plant)
Drosophila
Embryonic Development
embryogenesis
Embryonic Structures
metabolome
Amino Acids
Trehalose
Organic acids
metabolites
Metabolism
Sugars
Sugar Acids
Developmental Biology
Molecular weight
Carbohydrates

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Metabolome analysis of Drosophila melanogaster during embryogenesis. / Thuy An, Phan Nguyen; Yamaguchi, Masamitsu; Bamba, Takeshi; Fukusaki, Eiichiro.

In: PloS one, Vol. 9, No. 8, e99519, 14.08.2014.

Research output: Contribution to journalArticle

Thuy An, Phan Nguyen ; Yamaguchi, Masamitsu ; Bamba, Takeshi ; Fukusaki, Eiichiro. / Metabolome analysis of Drosophila melanogaster during embryogenesis. In: PloS one. 2014 ; Vol. 9, No. 8.
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