Metabolomic differential display analysis of the white-rot basidiomycete Phanerochaete chrysosporium grown under air and 100% oxygen

Daisuke Miura, Hiroo Tanaka, Hiroyuki Wariishi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

When the cultural atmosphere of the white-rot basidiomycete, Phanerochaete chrysosporium, was changed from air to 100% oxygen, the lyophilized mycelial weight increased and thickening of extracellular glucan layer was observed in 2-3 days. To better understand the oxygen-stress responsive mechanism of P. chrysosporium, the metabolomic differential display analysis was performed using metabolites isolated from fungal cells grown under either air or 100% O 2 atmosphere. In the GC-MS total ion chromatogram of methanol-extracts from fungal cells, at least 183 peaks were detected and 53 compounds were identified. Among them, veratryl alcohol (VA), threonate, and erythronate were identified as oxygen-stress responsive metabolites. The intracellular concentration of VA increased dramatically within 1 h after an oxygen purge, indicating that VA production is sensitive to the oxygen stress in P. chrysosporium.

Original languageEnglish
Pages (from-to)111-116
Number of pages6
JournalFEMS microbiology letters
Volume234
Issue number1
DOIs
Publication statusPublished - May 1 2004

Fingerprint

Phanerochaete
Basidiomycota
Metabolomics
Air
Oxygen
Atmosphere
Glucans
Cell Extracts
Methanol
Ions
Weights and Measures
veratryl alcohol

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Metabolomic differential display analysis of the white-rot basidiomycete Phanerochaete chrysosporium grown under air and 100% oxygen. / Miura, Daisuke; Tanaka, Hiroo; Wariishi, Hiroyuki.

In: FEMS microbiology letters, Vol. 234, No. 1, 01.05.2004, p. 111-116.

Research output: Contribution to journalArticle

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