TY - JOUR
T1 - Metal Complex Lipids for Fluid–Fluid Phase Separation in Coassembled Phospholipid Membranes
AU - Ohtani, Ryo
AU - Anegawa, Yuka
AU - Watanabe, Hikaru
AU - Tajima, Yutaro
AU - Kinoshita, Masanao
AU - Matsumori, Nobuaki
AU - Kawano, Kenichi
AU - Yanaka, Saeko
AU - Kato, Koichi
AU - Nakamura, Masaaki
AU - Ohba, Masaaki
AU - Hayami, Shinya
N1 - Funding Information:
This work was supported by JSPS Grant‐in‐Aid for Young Scientists JP18K14245 and JSPS Grant‐in‐Aid for Scientific Research on Innovative Areas, Mixed Anion JP19H04701. This work was also supported by KAKENHI Grant‐in‐Aid for Scientific Research (A) JP17H01200. This work was partially supported by the Cooperative Research Program of “Network Joint Research Centre for Materials and Devices”, and Kyushu Synchrotron Light Research Center (SAGA‐LS) 1807056F. This research was supported by Joint Research by Institute for Molecular Science (IMS) (IMS program No.101, 102, and 20–101). We thank Prof. Hayato Ishikawa and Mr. Kenta Rakumitsu (Kumamoto university) for the synthesis of and writing of the organic synthesis section. HCC‐L1
Funding Information:
This work was supported by JSPS Grant-in-Aid for Young Scientists JP18K14245 and JSPS Grant-in-Aid for Scientific Research on Innovative Areas, Mixed Anion JP19H04701. This work was also supported by KAKENHI Grant-in-Aid for Scientific Research (A) JP17H01200. This work was partially supported by the Cooperative Research Program of ?Network Joint Research Centre for Materials and Devices?, and Kyushu Synchrotron Light Research Center (SAGA-LS) 1807056F. This research was supported by Joint Research by Institute for Molecular Science (IMS) (IMS program No.101, 102, and 20?101). We thank Prof. Hayato Ishikawa and Mr. Kenta Rakumitsu (Kumamoto university) for the synthesis of HCC-L1 and writing of the organic synthesis section.
Publisher Copyright:
© 2021 Wiley-VCH GmbH
PY - 2021/6/7
Y1 - 2021/6/7
N2 - We demonstrate a fluid–fluid phase separation in 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes using a metal complex lipid of type [Mn(L1)] (1; HL1=1-(2-hydroxybenzamide)-2-(2-hydroxy-3-formyl-5-hexadecyloxybenzylideneamino)ethane). Small amount of 1 produces two separated domains in DMPC, whose phase transition temperatures of lipids (Tc) are both lower than that of the pristine DMPC. Variable temperature fluorescent microscopy for giant-unilamellar vesicles of DMPC/1 hybrids demonstrates that visible phase separations remain in fluid phases up to 37 °C, which is clearly over the Tc of DMPC. This provides a new dimension for the application of metal complex lipids toward controlling lipid distributions in fluid membranes.
AB - We demonstrate a fluid–fluid phase separation in 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes using a metal complex lipid of type [Mn(L1)] (1; HL1=1-(2-hydroxybenzamide)-2-(2-hydroxy-3-formyl-5-hexadecyloxybenzylideneamino)ethane). Small amount of 1 produces two separated domains in DMPC, whose phase transition temperatures of lipids (Tc) are both lower than that of the pristine DMPC. Variable temperature fluorescent microscopy for giant-unilamellar vesicles of DMPC/1 hybrids demonstrates that visible phase separations remain in fluid phases up to 37 °C, which is clearly over the Tc of DMPC. This provides a new dimension for the application of metal complex lipids toward controlling lipid distributions in fluid membranes.
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U2 - 10.1002/anie.202102774
DO - 10.1002/anie.202102774
M3 - Article
C2 - 33723910
AN - SCOPUS:85105312448
SN - 1433-7851
VL - 60
SP - 13603
EP - 13608
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 24
ER -