Metal mesh device sensor immobilized with a trimethoxysilane-containing glycopolymer for label-free detection of proteins and bacteria

Hirokazu Seto, Seiji Kamba, Takashi Kondo, Makoto Hasegawa, Shigeki Nashima, Yoshinobu Ehara, Yuichi Ogawa, Yu Hoshino, Yoshiko Miura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Biosensors for the detection of proteins and bacteria have been developed using glycopolymer-immobilized metal mesh devices. The trimethoxysilane- containing glycopolymer was immobilized onto a metal mesh device using the silane coupling reaction. The surface shape and transmittance properties of the original metal mesh device were maintained following the immobilization of the glycopolymer. The mannose-binding protein (concanavalin A) could be detected at concentrations in the range of 10-9 to 10-6 mol L -1 using the glycopolymer-immobilized metal mesh device sensor, whereas another protein (bovine serum albumin) was not detected. A detection limit of 1 ng mm-2 was achieved for the amount of adsorbed concanavalin A. The glycopolymer-immobilized metal mesh device sensor could also detect bacteria as well as protein. The mannose-binding strain of Escherichia coli was specifically detected by the glycopolymer-immobilized metal mesh device sensor. The glycopolymer-immobilized metal mesh device could therefore be used as a label-free biosensor showing high levels of selectivity and sensitivity toward proteins and bacteria.

Original languageEnglish
Pages (from-to)13234-13241
Number of pages8
JournalACS Applied Materials and Interfaces
Volume6
Issue number15
DOIs
Publication statusPublished - Aug 13 2014

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Fingerprint Dive into the research topics of 'Metal mesh device sensor immobilized with a trimethoxysilane-containing glycopolymer for label-free detection of proteins and bacteria'. Together they form a unique fingerprint.

  • Cite this