Abstract
Background/Aim: Metformin use has previously been associated with decreased cancer risk. The Hedgehog signaling pathway is a well-characterized early and late mediator of pancreatic cancer oncogenesis. The aim of the present study was to clarify the effect of metformin on factors involved in Hedgehog signaling. Materials and Methods: BxPC3 human pancreatic cancer cells were treated with metformin, and Sonic hedgehog (Shh) mRNA and protein levels were examined by real time reverse transcriptionpolymerase chain reaction, immunohistochemistry and immunoblotting, respectively. The effect of metformin on Shh levels was also examined in three other cancer cell lines. Results: Shh protein and mRNA expression was suppressed by metformin in BxPC3 cells. This phenomenon was further confirmed in three other cancer cell lines. Shh mRNA expression was inhibited by metformin in a concentrationdependent manner in two cancer cell lines. Conclusion: Metformin reduces the expression of Shh in several cancer cell lines including pancreatic cancer cell.
| Original language | English |
|---|---|
| Pages (from-to) | 1765-1770 |
| Number of pages | 6 |
| Journal | Anticancer research |
| Volume | 34 |
| Issue number | 4 |
| Publication status | Published - Apr 1 2014 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Cancer Research
Cite this
Metformin suppresses sonic hedgehog expression in pancreatic cancer cells. / Nakamura, Masafumi; Ogo, Ayako; Yamura, Masahiro; Yamaguchi, Yoshiyuki; Nakashima, Hiroshi.
In: Anticancer research, Vol. 34, No. 4, 01.04.2014, p. 1765-1770.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Metformin suppresses sonic hedgehog expression in pancreatic cancer cells
AU - Nakamura, Masafumi
AU - Ogo, Ayako
AU - Yamura, Masahiro
AU - Yamaguchi, Yoshiyuki
AU - Nakashima, Hiroshi
PY - 2014/4/1
Y1 - 2014/4/1
N2 - Background/Aim: Metformin use has previously been associated with decreased cancer risk. The Hedgehog signaling pathway is a well-characterized early and late mediator of pancreatic cancer oncogenesis. The aim of the present study was to clarify the effect of metformin on factors involved in Hedgehog signaling. Materials and Methods: BxPC3 human pancreatic cancer cells were treated with metformin, and Sonic hedgehog (Shh) mRNA and protein levels were examined by real time reverse transcriptionpolymerase chain reaction, immunohistochemistry and immunoblotting, respectively. The effect of metformin on Shh levels was also examined in three other cancer cell lines. Results: Shh protein and mRNA expression was suppressed by metformin in BxPC3 cells. This phenomenon was further confirmed in three other cancer cell lines. Shh mRNA expression was inhibited by metformin in a concentrationdependent manner in two cancer cell lines. Conclusion: Metformin reduces the expression of Shh in several cancer cell lines including pancreatic cancer cell.
AB - Background/Aim: Metformin use has previously been associated with decreased cancer risk. The Hedgehog signaling pathway is a well-characterized early and late mediator of pancreatic cancer oncogenesis. The aim of the present study was to clarify the effect of metformin on factors involved in Hedgehog signaling. Materials and Methods: BxPC3 human pancreatic cancer cells were treated with metformin, and Sonic hedgehog (Shh) mRNA and protein levels were examined by real time reverse transcriptionpolymerase chain reaction, immunohistochemistry and immunoblotting, respectively. The effect of metformin on Shh levels was also examined in three other cancer cell lines. Results: Shh protein and mRNA expression was suppressed by metformin in BxPC3 cells. This phenomenon was further confirmed in three other cancer cell lines. Shh mRNA expression was inhibited by metformin in a concentrationdependent manner in two cancer cell lines. Conclusion: Metformin reduces the expression of Shh in several cancer cell lines including pancreatic cancer cell.
UR - http://www.scopus.com/inward/record.url?scp=84902290137&partnerID=8YFLogxK
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M3 - Article
C2 - 24692708
AN - SCOPUS:84902290137
VL - 34
SP - 1765
EP - 1770
JO - Anticancer Research
JF - Anticancer Research
SN - 0250-7005
IS - 4
ER -