Methane production from food waste via mesophilic anaerobic digestion with ethanol pre-fermentation: Methanogenic pathway and microbial community analyses

Hui Zou, Ming Gao, Miao Yu, Wenyu Zhang, Shuang Zhang, Chuanfu Wu, Yukihiro Tashiro, Qunhui Wang

Research output: Contribution to journalArticle

Abstract

To investigate the methanogenic pathway and microbial community in a mesophilic anaerobic digestion (AD) system with food waste (FW) ethanol pre-fermentation (EP), two semi-continuous AD systems were operated by feeding FW with (PSR) and without EP (control). In this study, δ13C-ethanol was supplemented as solo substrate for AD sludge when the reactors operation stabilized to analyze the methanogenic pathways. The results suggested that approximately 59.3% of methane was produced from acetotrophic methanogens, while 40.7% was formed by hydrogenotrophic methanogens in the PSR group. On the other hand, compared with control, methane produced via CO2 reduction pathway was increased by 4.70%. Meanwhile, the composition variations of the microbial community in AD supported the above conclusion, since the relative abundances of Clostridium and Methanobacterium were enhanced by 7.6% and 10.2%, respectively in PSR reactor. These results provided a theoretical basis for AD applications and biogas yield improvements with EP process.

Original languageEnglish
Article number122450
JournalBioresource Technology
Volume297
DOIs
Publication statusPublished - Feb 2020

Fingerprint

Anaerobic digestion
Methane
Fermentation
fermentation
microbial community
ethanol
Ethanol
methane
food
Methanogens
Reactor operation
Clostridium
Biofuels
Biogas
biogas
relative abundance
sludge
anaerobic digestion
substrate
Substrates

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

Cite this

Methane production from food waste via mesophilic anaerobic digestion with ethanol pre-fermentation : Methanogenic pathway and microbial community analyses. / Zou, Hui; Gao, Ming; Yu, Miao; Zhang, Wenyu; Zhang, Shuang; Wu, Chuanfu; Tashiro, Yukihiro; Wang, Qunhui.

In: Bioresource Technology, Vol. 297, 122450, 02.2020.

Research output: Contribution to journalArticle

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AU - Wu, Chuanfu

AU - Tashiro, Yukihiro

AU - Wang, Qunhui

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