Method for measuring the three-dimensional distribution of a fluorescent dye in a cell membrane

Kazuya Yamamoto, Ichirou Ishimaru, Yoshiki Fujii, Toshiki Yasokawa, Shigeki Kuriyama, Tsutomu Masaki, Kaoru Takegawa, Naotaka Tanaka

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

This letter reports on a method for accurately determining the component distribution in a cell membrane over the entire cell surface. This method involves exciting a fluorescent-dyed cell membrane using evanescent light and scanning the entire cell surface by rotating the cell using a noncontact technique, namely, proximal two-beam optical tweezers. To position the cell membrane in the thin evanescent field, the authors designed an optical system capable of precisely positioning the focal position. Using this method, they were able to measure the surface distribution of glycoprotein labeled by lectin in a breast cancer cell membrane.

Original languageEnglish
Article number021110
JournalApplied Physics Letters
Volume90
Issue number2
DOIs
Publication statusPublished - 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

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