Method for measuring the three-dimensional distribution of a fluorescent dye in a cell membrane

Kazuya Yamamoto, Ichirou Ishimaru, Yoshiki Fujii, Toshiki Yasokawa, Shigeki Kuriyama, Tsutomu Masaki, Kaoru Takegawa, Naotaka Tanaka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

This letter reports on a method for accurately determining the component distribution in a cell membrane over the entire cell surface. This method involves exciting a fluorescent-dyed cell membrane using evanescent light and scanning the entire cell surface by rotating the cell using a noncontact technique, namely, proximal two-beam optical tweezers. To position the cell membrane in the thin evanescent field, the authors designed an optical system capable of precisely positioning the focal position. Using this method, they were able to measure the surface distribution of glycoprotein labeled by lectin in a breast cancer cell membrane.

Original languageEnglish
Article number021110
JournalApplied Physics Letters
Volume90
Issue number2
DOIs
Publication statusPublished - Jan 22 2007
Externally publishedYes

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dyes
cells
breast
positioning
cancer
scanning

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

Cite this

Yamamoto, K., Ishimaru, I., Fujii, Y., Yasokawa, T., Kuriyama, S., Masaki, T., ... Tanaka, N. (2007). Method for measuring the three-dimensional distribution of a fluorescent dye in a cell membrane. Applied Physics Letters, 90(2), [021110]. https://doi.org/10.1063/1.2428457

Method for measuring the three-dimensional distribution of a fluorescent dye in a cell membrane. / Yamamoto, Kazuya; Ishimaru, Ichirou; Fujii, Yoshiki; Yasokawa, Toshiki; Kuriyama, Shigeki; Masaki, Tsutomu; Takegawa, Kaoru; Tanaka, Naotaka.

In: Applied Physics Letters, Vol. 90, No. 2, 021110, 22.01.2007.

Research output: Contribution to journalArticle

Yamamoto, K, Ishimaru, I, Fujii, Y, Yasokawa, T, Kuriyama, S, Masaki, T, Takegawa, K & Tanaka, N 2007, 'Method for measuring the three-dimensional distribution of a fluorescent dye in a cell membrane', Applied Physics Letters, vol. 90, no. 2, 021110. https://doi.org/10.1063/1.2428457
Yamamoto K, Ishimaru I, Fujii Y, Yasokawa T, Kuriyama S, Masaki T et al. Method for measuring the three-dimensional distribution of a fluorescent dye in a cell membrane. Applied Physics Letters. 2007 Jan 22;90(2). 021110. https://doi.org/10.1063/1.2428457
Yamamoto, Kazuya ; Ishimaru, Ichirou ; Fujii, Yoshiki ; Yasokawa, Toshiki ; Kuriyama, Shigeki ; Masaki, Tsutomu ; Takegawa, Kaoru ; Tanaka, Naotaka. / Method for measuring the three-dimensional distribution of a fluorescent dye in a cell membrane. In: Applied Physics Letters. 2007 ; Vol. 90, No. 2.
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