Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences

Yuichi Kawanishi; Yutaka Banno; Hirofumi Fujimoto; Si Kab Nho; Zhenli Tu; Kazuei Mita; Kozo Tsuchida; Naoko Takada; Hideaki Maekawa; Yumiko Nakajima

Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences

Yuichi Kawanishi, Yutaka Banno, Hirofumi Fujimoto, Si Kab Nho, Zhenli Tu, Kazuei Mita, Kozo Tsuchida, Naoko Takada, Hideaki Maekawa, Yumiko Nakajima

Systems Biology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan) (Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.

Original language	English
Pages (from-to)	79-85
Number of pages	7
Journal	Journal of Insect Biotechnology and Sericology
Volume	77
Issue number	2
Publication status	Published - Jun 1 2008

All Science Journal Classification (ASJC) codes

Biotechnology
Applied Microbiology and Biotechnology
Business, Management and Accounting(all)
Agricultural and Biological Sciences(all)
Insect Science
Industrial and Manufacturing Engineering

Cite this

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title = "Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences",

abstract = "The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan) (Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.",

author = "Yuichi Kawanishi and Yutaka Banno and Hirofumi Fujimoto and Nho, {Si Kab} and Zhenli Tu and Kazuei Mita and Kozo Tsuchida and Naoko Takada and Hideaki Maekawa and Yumiko Nakajima",

year = "2008",

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language = "English",

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T1 - Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences

AU - Kawanishi, Yuichi

AU - Banno, Yutaka

AU - Fujimoto, Hirofumi

AU - Nho, Si Kab

AU - Tu, Zhenli

AU - Mita, Kazuei

AU - Tsuchida, Kozo

AU - Takada, Naoko

AU - Maekawa, Hideaki

AU - Nakajima, Yumiko

PY - 2008/6/1

Y1 - 2008/6/1

N2 - The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan) (Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.

AB - The EcoRI site is located between the 5.8S and 28S rRNA genes in Bombyx mandarina (Japan) (Japanese type rDNA) but is absent in the rDNA of B. mandarina (China)(B. mori type rDNA) including the domesticated B. mori. Digestion of PCR products amplified by primers of both flanking sides of this EcoRI site with EcoRI should only be successful for amplification products of B. mandarina (Japan) containing Japanese type rDNA. The distribution of Japanese type rDNA in Japan, Korea, and China was determined using this rapid detection method. Japanese type was not detected in China or in Korea despite having 27 chromosomes. These findings suggest that the choromosome fusion and the rDNA type separation were independent evolutionary events. Invasion of B. mori chromosome #11 into Japanese B. mandarina (Japan) was examined.

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VL - 77

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JF - Journal of Insect Biotechnology and Sericology

IS - 2

ER -

Method for rapid distinction of Bombyx mandarina (Japan) from B. mandarina (China) based on rDNA sequence differences

Abstract

All Science Journal Classification (ASJC) codes

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