Methodologies and strategies for the bioengineering of lantibiotics

Jun ichi Nagao, Mami Nishie, Kenji Sonomoto

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Lantibiotics are ribosomally synthesized, post-translationally modified, peptide antibiotics containing unusual amino acids such as dehydrated amino acids and lanthionine. These unusual amino acids impose conformational constraints on the peptide and contribute to the biological activity and high physicochemical stability of lantibiotics. Recent researches on the modification enzymes responsible for dehydration and cyclization have considerably increased our understanding of their molecular characteristics and relaxed specificity. These insights enabled us to exploit these modification enzymes for developing new lantibiotic variants with improved therapeutic potential. Several methodologies have been explored to engineer novel lantibiotics. Here, we outline the current knowledge of modification enzymes. We also describe the methodologies and strategies used to engineer lantibiotics and provide some examples of successful generation of lantibiotics with enhanced activity.

Original languageEnglish
Pages (from-to)1221-1230
Number of pages10
JournalCurrent Pharmaceutical Biotechnology
Volume12
Issue number8
DOIs
Publication statusPublished - Aug 2011

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Biotechnology

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