Methylation of a conserved intronic CpG island of mouse SF-1 is associated with cell-specific expression of SF-1 in a culture system but not with tissue-specific expression

Hisao Shirohzu, Taijiro Okabe, Shigeki Gondo, Tomoko Tanaka, Kenji Ohe, Hidetaka Morinaga, Hisaya Kawate, Masatoshi Nomura, Ryoichi Takayanagi, Hajime Nawata, Toshihiko Yanase

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Abstract

The mechanism for the steroidogenic tissue or cell-specific expression of SF-1 has not been well clarified. We examined whether the methylation status of a large CpG island in the first intron of mouse SF-1 gene is associated with the expression level of SF-1 in cultured cells and in tissues. The island consists of three small islands (ICI-1, ICI-2, and ICI-3). In cultured adrenocortical Y-1 cells and in Leydig tumor cells, I-10, that both express high levels of SF-1, the upstream region of ICI-2, ICI-2-1, was clearly hypomethylated compared to cultured mouse bone marrow cells that do not express SF-1. However, this methylation status was not clearly associated with the tissue-specific expression of SF-1, in either adult or during development. These results suggest that methylation of ICI-2-1of SF-1 may partly determine the level of SF-1 expression at the cellular level, but may not be essential at the tissue level.

Original languageEnglish
Pages (from-to)862-867
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume369
Issue number3
DOIs
Publication statusPublished - May 9 2008

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All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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