Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein–Barr virus-associated gastric cancer

Hirofumi Ohmura; Mamoru Ito; Keita Uchino; Chihiro Okada; Shigeki Tanishima; Yuichi Yamada; Seiya Momosaki; Masato Komoda; Miyuki Kuwayama; Kyoko Yamaguchi; Yuta Okumura; Michitaka Nakano; Kenji Tsuchihashi; Taichi Isobe; Hiroshi Ariyama; Hitoshi Kusaba; Yoshinao Oda; Koichi Akashi; Eishi Baba

doi:10.1002/2211-5463.12765

Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein–Barr virus-associated gastric cancer

Hirofumi Ohmura, Mamoru Ito, Keita Uchino, Chihiro Okada, Shigeki Tanishima, Yuichi Yamada, Seiya Momosaki, Masato Komoda, Miyuki Kuwayama, Kyoko Yamaguchi, Yuta Okumura, Michitaka Nakano, Kenji Tsuchihashi, Taichi Isobe, Hiroshi Ariyama, Hitoshi Kusaba, Yoshinao Oda, Koichi Akashi, Eishi Baba

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Epstein–Barr virus (EBV)-associated gastric cancer (GC) is associated with a high degree of DNA methylation. However, the association between chemotherapy susceptibility and tumor DNA methylation in advanced diseases remains unclear. The comprehensive DNA methylation status of GC cells obtained from an advanced EBV-associated GC (EBVGC) case, in which complete response to S-1 plus cisplatin chemotherapy was achieved, was analyzed using a DNA methylation microarray. We compared DNA methylation of GC cells with public data and identified genes with higher methylation in EBVGC cell lines than in normal gastric cells, and genes in which methylation was increased by EBV. Of these genes, ABCG2, AHNAK2, BCL2, FZD1, and TP73 are associated with published evidence for resistance to 5-fluorouracil and cisplatin. Silencing of these genes may be associated with hypersensitivity to chemotherapy.

Original language	English
Pages (from-to)	147-157
Number of pages	11
Journal	FEBS Open Bio
Volume	10
Issue number	1
DOIs	https://doi.org/10.1002/2211-5463.12765
Publication status	Published - Jan 1 2020

All Science Journal Classification (ASJC) codes

Biochemistry, Genetics and Molecular Biology(all)

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Ohmura, H., Ito, M., Uchino, K., Okada, C., Tanishima, S., Yamada, Y., Momosaki, S., Komoda, M., Kuwayama, M., Yamaguchi, K., Okumura, Y., Nakano, M., Tsuchihashi, K., Isobe, T., Ariyama, H., Kusaba, H., Oda, Y., Akashi, K., & Baba, E. (2020). Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein–Barr virus-associated gastric cancer. FEBS Open Bio, 10(1), 147-157. https://doi.org/10.1002/2211-5463.12765

Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein–Barr virus-associated gastric cancer. / Ohmura, Hirofumi; Ito, Mamoru; Uchino, Keita; Okada, Chihiro; Tanishima, Shigeki; Yamada, Yuichi; Momosaki, Seiya; Komoda, Masato; Kuwayama, Miyuki; Yamaguchi, Kyoko; Okumura, Yuta; Nakano, Michitaka; Tsuchihashi, Kenji ; Isobe, Taichi ; Ariyama, Hiroshi ; Kusaba, Hitoshi ; Oda, Yoshinao ; Akashi, Koichi ; Baba, Eishi.

In: FEBS Open Bio, Vol. 10, No. 1, 01.01.2020, p. 147-157.

Research output: Contribution to journal › Article › peer-review

Ohmura, H, Ito, M, Uchino, K, Okada, C, Tanishima, S, Yamada, Y, Momosaki, S, Komoda, M, Kuwayama, M, Yamaguchi, K, Okumura, Y, Nakano, M, Tsuchihashi, K , Isobe, T , Ariyama, H , Kusaba, H , Oda, Y , Akashi, K & Baba, E 2020, 'Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein–Barr virus-associated gastric cancer', FEBS Open Bio, vol. 10, no. 1, pp. 147-157. https://doi.org/10.1002/2211-5463.12765

Ohmura, Hirofumi ; Ito, Mamoru ; Uchino, Keita ; Okada, Chihiro ; Tanishima, Shigeki ; Yamada, Yuichi ; Momosaki, Seiya ; Komoda, Masato ; Kuwayama, Miyuki ; Yamaguchi, Kyoko ; Okumura, Yuta ; Nakano, Michitaka ; Tsuchihashi, Kenji ; Isobe, Taichi ; Ariyama, Hiroshi ; Kusaba, Hitoshi ; Oda, Yoshinao ; Akashi, Koichi ; Baba, Eishi. / Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein–Barr virus-associated gastric cancer. In: FEBS Open Bio. 2020 ; Vol. 10, No. 1. pp. 147-157.

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abstract = "Epstein–Barr virus (EBV)-associated gastric cancer (GC) is associated with a high degree of DNA methylation. However, the association between chemotherapy susceptibility and tumor DNA methylation in advanced diseases remains unclear. The comprehensive DNA methylation status of GC cells obtained from an advanced EBV-associated GC (EBVGC) case, in which complete response to S-1 plus cisplatin chemotherapy was achieved, was analyzed using a DNA methylation microarray. We compared DNA methylation of GC cells with public data and identified genes with higher methylation in EBVGC cell lines than in normal gastric cells, and genes in which methylation was increased by EBV. Of these genes, ABCG2, AHNAK2, BCL2, FZD1, and TP73 are associated with published evidence for resistance to 5-fluorouracil and cisplatin. Silencing of these genes may be associated with hypersensitivity to chemotherapy.",

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AU - Tanishima, Shigeki

AU - Yamada, Yuichi

AU - Momosaki, Seiya

AU - Komoda, Masato

AU - Kuwayama, Miyuki

AU - Yamaguchi, Kyoko

AU - Okumura, Yuta

AU - Nakano, Michitaka

AU - Tsuchihashi, Kenji

AU - Isobe, Taichi

AU - Ariyama, Hiroshi

AU - Kusaba, Hitoshi

AU - Oda, Yoshinao

AU - Akashi, Koichi

AU - Baba, Eishi

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