Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein–Barr virus-associated gastric cancer

Hirofumi Ohmura, Mamoru Ito, Keita Uchino, Chihiro Okada, Shigeki Tanishima, Yuichi Yamada, Seiya Momosaki, Masato Komoda, Miyuki Kuwayama, Kyoko Yamaguchi, Yuta Okumura, Michitaka Nakano, Kenji Tsuchihashi, Taichi Isobe, Hiroshi Ariyama, Hitoshi Kusaba, Yoshinao Oda, Koichi Akashi, Eishi Baba

Research output: Contribution to journalArticle

Abstract

Epstein–Barr virus (EBV)-associated gastric cancer (GC) is associated with a high degree of DNA methylation. However, the association between chemotherapy susceptibility and tumor DNA methylation in advanced diseases remains unclear. The comprehensive DNA methylation status of GC cells obtained from an advanced EBV-associated GC (EBVGC) case, in which complete response to S-1 plus cisplatin chemotherapy was achieved, was analyzed using a DNA methylation microarray. We compared DNA methylation of GC cells with public data and identified genes with higher methylation in EBVGC cell lines than in normal gastric cells, and genes in which methylation was increased by EBV. Of these genes, ABCG2, AHNAK2, BCL2, FZD1, and TP73 are associated with published evidence for resistance to 5-fluorouracil and cisplatin. Silencing of these genes may be associated with hypersensitivity to chemotherapy.

Original languageEnglish
Pages (from-to)147-157
Number of pages11
JournalFEBS Open Bio
Volume10
Issue number1
DOIs
Publication statusPublished - Jan 1 2020

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Methylation
Chemotherapy
DNA Methylation
Viruses
Drug Resistance
Stomach Neoplasms
Genes
Drug Therapy
Pharmaceutical Preparations
Cisplatin
Gene Silencing
Microarrays
Oligonucleotide Array Sequence Analysis
Human Herpesvirus 4
Fluorouracil
Tumors
Stomach
Hypersensitivity
Cells
Association reactions

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein–Barr virus-associated gastric cancer. / Ohmura, Hirofumi; Ito, Mamoru; Uchino, Keita; Okada, Chihiro; Tanishima, Shigeki; Yamada, Yuichi; Momosaki, Seiya; Komoda, Masato; Kuwayama, Miyuki; Yamaguchi, Kyoko; Okumura, Yuta; Nakano, Michitaka; Tsuchihashi, Kenji; Isobe, Taichi; Ariyama, Hiroshi; Kusaba, Hitoshi; Oda, Yoshinao; Akashi, Koichi; Baba, Eishi.

In: FEBS Open Bio, Vol. 10, No. 1, 01.01.2020, p. 147-157.

Research output: Contribution to journalArticle

Ohmura, H, Ito, M, Uchino, K, Okada, C, Tanishima, S, Yamada, Y, Momosaki, S, Komoda, M, Kuwayama, M, Yamaguchi, K, Okumura, Y, Nakano, M, Tsuchihashi, K, Isobe, T, Ariyama, H, Kusaba, H, Oda, Y, Akashi, K & Baba, E 2020, 'Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein–Barr virus-associated gastric cancer', FEBS Open Bio, vol. 10, no. 1, pp. 147-157. https://doi.org/10.1002/2211-5463.12765
Ohmura, Hirofumi ; Ito, Mamoru ; Uchino, Keita ; Okada, Chihiro ; Tanishima, Shigeki ; Yamada, Yuichi ; Momosaki, Seiya ; Komoda, Masato ; Kuwayama, Miyuki ; Yamaguchi, Kyoko ; Okumura, Yuta ; Nakano, Michitaka ; Tsuchihashi, Kenji ; Isobe, Taichi ; Ariyama, Hiroshi ; Kusaba, Hitoshi ; Oda, Yoshinao ; Akashi, Koichi ; Baba, Eishi. / Methylation of drug resistance-related genes in chemotherapy-sensitive Epstein–Barr virus-associated gastric cancer. In: FEBS Open Bio. 2020 ; Vol. 10, No. 1. pp. 147-157.
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