MHC class I bound peptides of a colon carcinoma cell line, a Ki-ras gene-targeted progeny cell line and a B cell line

MHC class I associated peptides on cancer cells represent potential targets for CD8⁺ cytotoxic T cell activity against tumor cells. We eluted the naturally bound MHC class I peptides of a colon carcinoma cell line and compared them to peptides isolated from a B cell line and a slow-growing activated Ki-ras-disrupted colon cancer cell line. While we failed to detect any significant differences in class I associated peptides due to the presence or absence of activated Ki-ras in colon cancer cell lines, the colon cancer cell lines and B cell line presented vastly different peptide repertoires in the context of HLA-A*0201 molecules.