Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions

Mai Yamamoto, Ryoko Morita, Takamasa Mizoguchi, Hiromi Matsuo, Miho Isoda, Tohru Ishitani, Ajay B. Chitnis, Kunihiro Matsumoto, J. Gage Crump, Katsuto Hozumi, Shigenobu Yonemura, Koichi Kawakami, Motoyuki Itoh

Research output: Contribution to journalArticle

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Abstract

In the developing embryo, cell-cell signalling is necessary for tissue patterning and structural organization. During midline development, the notochord plays roles in the patterning of its surrounding tissues while forming the axial structure; however, how these patterning and structural roles are coordinated remains elusive. Here, we identify a mechanism by which Notch signalling regulates the patterning activities and structural integrity of the notochord. We found that Mind bomb (Mib) ubiquitylates Jagged 1 (Jag1) and is essential in the signal-emitting cells for Jag1 to activate Notch signalling. In zebrafish, loss- and gain-of-function analyses showed that Mib-Jag1-Notch signalling favours the development of non-vacuolated cells at the expense of vacuolated cells in the notochord. This leads to changes in the peri-notochordal basement membrane formation and patterning surrounding the muscle pioneer cells. These data reveal a previously unrecognized mechanism regulating the patterning and structural roles of the notochord by Mib-Jag1-Notch signalling-mediated cell-fate determination.

Original languageEnglish
Pages (from-to)2527-2537
Number of pages11
JournalDevelopment
Volume137
Issue number15
DOIs
Publication statusPublished - Aug 1 2010

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Bombs
Notochord
Zebrafish
Basement Membrane
Muscle Cells
Jagged-1 Protein
Embryonic Structures

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

Cite this

Yamamoto, M., Morita, R., Mizoguchi, T., Matsuo, H., Isoda, M., Ishitani, T., ... Itoh, M. (2010). Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions. Development, 137(15), 2527-2537. https://doi.org/10.1242/dev.051011

Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions. / Yamamoto, Mai; Morita, Ryoko; Mizoguchi, Takamasa; Matsuo, Hiromi; Isoda, Miho; Ishitani, Tohru; Chitnis, Ajay B.; Matsumoto, Kunihiro; Crump, J. Gage; Hozumi, Katsuto; Yonemura, Shigenobu; Kawakami, Koichi; Itoh, Motoyuki.

In: Development, Vol. 137, No. 15, 01.08.2010, p. 2527-2537.

Research output: Contribution to journalArticle

Yamamoto, M, Morita, R, Mizoguchi, T, Matsuo, H, Isoda, M, Ishitani, T, Chitnis, AB, Matsumoto, K, Crump, JG, Hozumi, K, Yonemura, S, Kawakami, K & Itoh, M 2010, 'Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions', Development, vol. 137, no. 15, pp. 2527-2537. https://doi.org/10.1242/dev.051011
Yamamoto, Mai ; Morita, Ryoko ; Mizoguchi, Takamasa ; Matsuo, Hiromi ; Isoda, Miho ; Ishitani, Tohru ; Chitnis, Ajay B. ; Matsumoto, Kunihiro ; Crump, J. Gage ; Hozumi, Katsuto ; Yonemura, Shigenobu ; Kawakami, Koichi ; Itoh, Motoyuki. / Mib-Jag1-Notch signalling regulates patterning and structural roles of the notochord by controlling cell-fate decisions. In: Development. 2010 ; Vol. 137, No. 15. pp. 2527-2537.
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