Mice lacking connexin45 conditionally in cardiac myocytes display embryonic lethality similar to that of germline knockout mice without endocardial cushion defect

Kiyomasa Nishii, Madoka Kumai, Katsuko Egashira, Takeshi Miwa, Kanako Hashizume, Yumi Miyano, Yosaburo Shibata

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The gap junction protein connexin45-deficient (Cx45-KO) mice die shortly after the hearts begin to beat. In addition to the heart defect, they also show defective vascular development which may be closely related with the cardiac phenotype. Therefore, we created mice whose floxed-Cx45 locus could be removed conditionally. We utilized cardiac α-actin-Cre transgenic mice to investigate the specific cardiac muscular function of Cx45 in vivo. The resultant conditional mutants were lethal, showing conduction block similar to that of the Cx45-KO mice. Unlike Cx45-KO, development of the endocardial cushion was not disrupted in the conditional mutants. X-gal staining was detected in the embryonic cardiac myocytes as a hallmark of Cre-loxP mediated floxed-Cx45 deletion. These results reconfirm the requirement of Cx45 for developing cardiac myocytes. These also indicate that establishing the first contractions is a crucial task for the early hearts.

Original languageEnglish
Pages (from-to)365-369
Number of pages5
JournalCell Communication and Adhesion
Volume10
Issue number4-6
Publication statusPublished - Jul 1 2003
Externally publishedYes

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Endocardial Cushion Defects
Cardiac Myocytes
Knockout Mice
Defects
Endocardial Cushions
Connexins
Transgenic Mice
Blood Vessels
Actins
Staining and Labeling
Phenotype

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry
  • Cell Biology

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Mice lacking connexin45 conditionally in cardiac myocytes display embryonic lethality similar to that of germline knockout mice without endocardial cushion defect. / Nishii, Kiyomasa; Kumai, Madoka; Egashira, Katsuko; Miwa, Takeshi; Hashizume, Kanako; Miyano, Yumi; Shibata, Yosaburo.

In: Cell Communication and Adhesion, Vol. 10, No. 4-6, 01.07.2003, p. 365-369.

Research output: Contribution to journalArticle

Nishii, Kiyomasa ; Kumai, Madoka ; Egashira, Katsuko ; Miwa, Takeshi ; Hashizume, Kanako ; Miyano, Yumi ; Shibata, Yosaburo. / Mice lacking connexin45 conditionally in cardiac myocytes display embryonic lethality similar to that of germline knockout mice without endocardial cushion defect. In: Cell Communication and Adhesion. 2003 ; Vol. 10, No. 4-6. pp. 365-369.
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