Mice lacking serum amyloid p component do not necessarily develop severe autoimmune disease

Makoto Soma, Toshio Tamaoki, Hiroo Kawano, Sadahiro Ito, Mihoko Sakamoto, Yoshiie Okada, Yukio Ozaki, Shigenobu Kanba, Yoshiki Hamada, Tokuhiro Ishihara, Shuichiro Maeda

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Abstract

Serum amyloid P component (SAP) is a major acute-phase reactant in mice. Recently, it was reported that SAP-deficient mice spontaneously developed antinuclear antibodies and severe glomerulonephritis. Because the SAP-deficient mice we generated display no obvious phenotypic abnormalities, we investigated whether our SAP-deficient mice would also spontaneously develop autoimmune responses. In accordance with the report, our mice produced high titers of antinuclear antibody but did not develop severe glomerulonephritis. On the other hand, it was recently reported that SAP bound to gram-negative bacteria via lipopolysaccharide (LPS) prevented LPS-mediated activation of a classical complement pathway. Thus, we asked if SAP-deficient mice would show altered responses to an intraperitoneal injection of LPS from Salmonella typhimirium. SAP-deficiency did afford resistance to lethality induced by high-dose LPS. Our experiments clearly showed that contrary to documented data, SAP-deficient mice do not develop serious autoimmune disease and we suggest that SAP has a critical role in LPS toxicity.

Original languageEnglish
Pages (from-to)200-205
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume286
Issue number1
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

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Serum Amyloid P-Component
Amyloid
Autoimmune Diseases
Serum
Lipopolysaccharides
Antinuclear Antibodies
Glomerulonephritis
Classical Complement Pathway
Salmonella
Acute-Phase Proteins
Intraperitoneal Injections
Gram-Negative Bacteria
Autoimmunity
Toxicity
Bacteria
Chemical activation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Mice lacking serum amyloid p component do not necessarily develop severe autoimmune disease. / Soma, Makoto; Tamaoki, Toshio; Kawano, Hiroo; Ito, Sadahiro; Sakamoto, Mihoko; Okada, Yoshiie; Ozaki, Yukio; Kanba, Shigenobu; Hamada, Yoshiki; Ishihara, Tokuhiro; Maeda, Shuichiro.

In: Biochemical and Biophysical Research Communications, Vol. 286, No. 1, 01.01.2001, p. 200-205.

Research output: Contribution to journalArticle

Soma, M, Tamaoki, T, Kawano, H, Ito, S, Sakamoto, M, Okada, Y, Ozaki, Y, Kanba, S, Hamada, Y, Ishihara, T & Maeda, S 2001, 'Mice lacking serum amyloid p component do not necessarily develop severe autoimmune disease', Biochemical and Biophysical Research Communications, vol. 286, no. 1, pp. 200-205. https://doi.org/10.1006/bbrc.2001.5364
Soma, Makoto ; Tamaoki, Toshio ; Kawano, Hiroo ; Ito, Sadahiro ; Sakamoto, Mihoko ; Okada, Yoshiie ; Ozaki, Yukio ; Kanba, Shigenobu ; Hamada, Yoshiki ; Ishihara, Tokuhiro ; Maeda, Shuichiro. / Mice lacking serum amyloid p component do not necessarily develop severe autoimmune disease. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 286, No. 1. pp. 200-205.
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