Mice that express human interleukin-8 have increased mobilization of immature myeloid cells, which exacerbates inflammation and accelerates colon carcinogenesis

Samuel Asfaha, Alexander N. Dubeykovskiy, Hiroyuki Tomita, Xiangdong Yang, Sarah Stokes, Wataru Shibata, Richard A. Friedman, hiroshi ariyama, Zinaida A. Dubeykovskaya, Sureshkumar Muthupalani, Russell Ericksen, Harold Frucht, James G. Fox, Timothy C. Wang

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Abstract

Background & Aims: Interleukin (IL)-8 has an important role in initiating inflammation in humans, attracting immune cells such as neutrophils through their receptors CXCR1 and CXCR2. IL-8 has been proposed to contribute to chronic inflammation and cancer. However, mice do not have the IL-8 gene, so human cancer cell lines and xenograft studies have been used to study the role of IL-8 in colon and gastric carcinogenesis. We generated mice that carry a bacterial artificial chromosome that encompasses the entire human IL-8 gene, including its regulatory elements (IL-8Tg mice). Methods: We studied the effects of IL-8 expression in APCmin+/- mice and IL-8Tg mice given azoxymethane and dextran sodium sulfate (DSS). We also examined the effects of IL-8 expression in gastric cancer in INS-GAS mice that overexpress gastrin and IL-8Tg mice infected with Helicobacter felis. Results: In IL-8Tg mice, expression of human IL-8 was controlled by its own regulatory elements, with virtually no messenger RNA or protein detectable under basal conditions. IL-8 was strongly up-regulated on systemic or local inflammatory stimulation, increasing mobilization of immature CD11b+Gr-1+ myeloid cells (IMCs) with thioglycolate-induced peritonitis, DSS-induced colitis, and H. felis-induced gastritis. IL-8 was increased in colorectal tumors from patients and IL-8Tg mice compared with nontumor tissues. IL-8Tg mice developed more tumors than wild-type mice following administration of azoxymethane and DSS. Expression of IL-8 increased tumorigenesis in APCmin+/- mice compared with APCmin+/- mice that lack IL-8; this was associated with increased numbers of IMCs and angiogenesis in the tumors. Conclusions: IL-8 contributes to gastrointestinal carcinogenesis by mobilizing IMCs and might be a therapeutic target for gastrointestinal cancers.

Original languageEnglish
Pages (from-to)155-166
Number of pages12
JournalGastroenterology
Volume144
Issue number1
DOIs
Publication statusPublished - Jan 1 2013

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Myeloid Cells
Interleukin-8
Colon
Carcinogenesis
Inflammation
Interleukins
Dextran Sulfate
Helicobacter felis
Azoxymethane
Interleukin-8A Receptors
Neoplasms
Interleukin-8B Receptors
Thioglycolates
Bacterial Artificial Chromosomes
Gastrointestinal Neoplasms
Gastrins
Gastritis
Colitis
Peritonitis
Heterografts

All Science Journal Classification (ASJC) codes

  • Hepatology
  • Gastroenterology

Cite this

Mice that express human interleukin-8 have increased mobilization of immature myeloid cells, which exacerbates inflammation and accelerates colon carcinogenesis. / Asfaha, Samuel; Dubeykovskiy, Alexander N.; Tomita, Hiroyuki; Yang, Xiangdong; Stokes, Sarah; Shibata, Wataru; Friedman, Richard A.; ariyama, hiroshi; Dubeykovskaya, Zinaida A.; Muthupalani, Sureshkumar; Ericksen, Russell; Frucht, Harold; Fox, James G.; Wang, Timothy C.

In: Gastroenterology, Vol. 144, No. 1, 01.01.2013, p. 155-166.

Research output: Contribution to journalArticle

Asfaha, S, Dubeykovskiy, AN, Tomita, H, Yang, X, Stokes, S, Shibata, W, Friedman, RA, ariyama, H, Dubeykovskaya, ZA, Muthupalani, S, Ericksen, R, Frucht, H, Fox, JG & Wang, TC 2013, 'Mice that express human interleukin-8 have increased mobilization of immature myeloid cells, which exacerbates inflammation and accelerates colon carcinogenesis', Gastroenterology, vol. 144, no. 1, pp. 155-166. https://doi.org/10.1053/j.gastro.2012.09.057
Asfaha, Samuel ; Dubeykovskiy, Alexander N. ; Tomita, Hiroyuki ; Yang, Xiangdong ; Stokes, Sarah ; Shibata, Wataru ; Friedman, Richard A. ; ariyama, hiroshi ; Dubeykovskaya, Zinaida A. ; Muthupalani, Sureshkumar ; Ericksen, Russell ; Frucht, Harold ; Fox, James G. ; Wang, Timothy C. / Mice that express human interleukin-8 have increased mobilization of immature myeloid cells, which exacerbates inflammation and accelerates colon carcinogenesis. In: Gastroenterology. 2013 ; Vol. 144, No. 1. pp. 155-166.
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T1 - Mice that express human interleukin-8 have increased mobilization of immature myeloid cells, which exacerbates inflammation and accelerates colon carcinogenesis

AU - Asfaha, Samuel

AU - Dubeykovskiy, Alexander N.

AU - Tomita, Hiroyuki

AU - Yang, Xiangdong

AU - Stokes, Sarah

AU - Shibata, Wataru

AU - Friedman, Richard A.

AU - ariyama, hiroshi

AU - Dubeykovskaya, Zinaida A.

AU - Muthupalani, Sureshkumar

AU - Ericksen, Russell

AU - Frucht, Harold

AU - Fox, James G.

AU - Wang, Timothy C.

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N2 - Background & Aims: Interleukin (IL)-8 has an important role in initiating inflammation in humans, attracting immune cells such as neutrophils through their receptors CXCR1 and CXCR2. IL-8 has been proposed to contribute to chronic inflammation and cancer. However, mice do not have the IL-8 gene, so human cancer cell lines and xenograft studies have been used to study the role of IL-8 in colon and gastric carcinogenesis. We generated mice that carry a bacterial artificial chromosome that encompasses the entire human IL-8 gene, including its regulatory elements (IL-8Tg mice). Methods: We studied the effects of IL-8 expression in APCmin+/- mice and IL-8Tg mice given azoxymethane and dextran sodium sulfate (DSS). We also examined the effects of IL-8 expression in gastric cancer in INS-GAS mice that overexpress gastrin and IL-8Tg mice infected with Helicobacter felis. Results: In IL-8Tg mice, expression of human IL-8 was controlled by its own regulatory elements, with virtually no messenger RNA or protein detectable under basal conditions. IL-8 was strongly up-regulated on systemic or local inflammatory stimulation, increasing mobilization of immature CD11b+Gr-1+ myeloid cells (IMCs) with thioglycolate-induced peritonitis, DSS-induced colitis, and H. felis-induced gastritis. IL-8 was increased in colorectal tumors from patients and IL-8Tg mice compared with nontumor tissues. IL-8Tg mice developed more tumors than wild-type mice following administration of azoxymethane and DSS. Expression of IL-8 increased tumorigenesis in APCmin+/- mice compared with APCmin+/- mice that lack IL-8; this was associated with increased numbers of IMCs and angiogenesis in the tumors. Conclusions: IL-8 contributes to gastrointestinal carcinogenesis by mobilizing IMCs and might be a therapeutic target for gastrointestinal cancers.

AB - Background & Aims: Interleukin (IL)-8 has an important role in initiating inflammation in humans, attracting immune cells such as neutrophils through their receptors CXCR1 and CXCR2. IL-8 has been proposed to contribute to chronic inflammation and cancer. However, mice do not have the IL-8 gene, so human cancer cell lines and xenograft studies have been used to study the role of IL-8 in colon and gastric carcinogenesis. We generated mice that carry a bacterial artificial chromosome that encompasses the entire human IL-8 gene, including its regulatory elements (IL-8Tg mice). Methods: We studied the effects of IL-8 expression in APCmin+/- mice and IL-8Tg mice given azoxymethane and dextran sodium sulfate (DSS). We also examined the effects of IL-8 expression in gastric cancer in INS-GAS mice that overexpress gastrin and IL-8Tg mice infected with Helicobacter felis. Results: In IL-8Tg mice, expression of human IL-8 was controlled by its own regulatory elements, with virtually no messenger RNA or protein detectable under basal conditions. IL-8 was strongly up-regulated on systemic or local inflammatory stimulation, increasing mobilization of immature CD11b+Gr-1+ myeloid cells (IMCs) with thioglycolate-induced peritonitis, DSS-induced colitis, and H. felis-induced gastritis. IL-8 was increased in colorectal tumors from patients and IL-8Tg mice compared with nontumor tissues. IL-8Tg mice developed more tumors than wild-type mice following administration of azoxymethane and DSS. Expression of IL-8 increased tumorigenesis in APCmin+/- mice compared with APCmin+/- mice that lack IL-8; this was associated with increased numbers of IMCs and angiogenesis in the tumors. Conclusions: IL-8 contributes to gastrointestinal carcinogenesis by mobilizing IMCs and might be a therapeutic target for gastrointestinal cancers.

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