Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark

Sayuri Arai, Mina Okochi, Taizo Hanai, Hiroyuki Honda

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Photosynthetic microorganisms such as cyanobacteria have attracted attention for their potential to produce biofuels and biochemicals directly from CO2. Cell isolation by colony has conventionally been used for selecting target cells. Colony isolation methods require a significant amount of time for cultivation, and the colony-forming ratio is potentially low for cyanobacteria. Here, we overcome such limitations by encapsulating and culturing cells in droplets with an overlay of dodecane using glass slides printed with highly water-repellent mark. In the compartmentalized culture, the oil phase protects the small volume of culture medium from drying and increases the CO2 supply. Since a difference in cell growth was observed with and without the addition of antibiotics, this compartmentalized culture method could be a powerful tool for mutant selection.

Original languageEnglish
Pages (from-to)151-155
Number of pages5
JournalBiotechnology Reports
Volume4
DOIs
Publication statusPublished - Dec 1 2014

Fingerprint

Cyanobacteria
Glass
Water
Biofuels
Cell Separation
Culture Media
Oils
Anti-Bacterial Agents
Growth

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark. / Arai, Sayuri; Okochi, Mina; Hanai, Taizo; Honda, Hiroyuki.

In: Biotechnology Reports, Vol. 4, 01.12.2014, p. 151-155.

Research output: Contribution to journalArticle

@article{6e39b1c3b0cb405095d90c418a11f1e0,
title = "Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark",
abstract = "Photosynthetic microorganisms such as cyanobacteria have attracted attention for their potential to produce biofuels and biochemicals directly from CO2. Cell isolation by colony has conventionally been used for selecting target cells. Colony isolation methods require a significant amount of time for cultivation, and the colony-forming ratio is potentially low for cyanobacteria. Here, we overcome such limitations by encapsulating and culturing cells in droplets with an overlay of dodecane using glass slides printed with highly water-repellent mark. In the compartmentalized culture, the oil phase protects the small volume of culture medium from drying and increases the CO2 supply. Since a difference in cell growth was observed with and without the addition of antibiotics, this compartmentalized culture method could be a powerful tool for mutant selection.",
author = "Sayuri Arai and Mina Okochi and Taizo Hanai and Hiroyuki Honda",
year = "2014",
month = "12",
day = "1",
doi = "10.1016/j.btre.2014.10.003",
language = "English",
volume = "4",
pages = "151--155",
journal = "Biotechnology Reports",
issn = "2215-017X",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Micro-compartmentalized cultivation of cyanobacteria for mutant screening using glass slides with highly water-repellent mark

AU - Arai, Sayuri

AU - Okochi, Mina

AU - Hanai, Taizo

AU - Honda, Hiroyuki

PY - 2014/12/1

Y1 - 2014/12/1

N2 - Photosynthetic microorganisms such as cyanobacteria have attracted attention for their potential to produce biofuels and biochemicals directly from CO2. Cell isolation by colony has conventionally been used for selecting target cells. Colony isolation methods require a significant amount of time for cultivation, and the colony-forming ratio is potentially low for cyanobacteria. Here, we overcome such limitations by encapsulating and culturing cells in droplets with an overlay of dodecane using glass slides printed with highly water-repellent mark. In the compartmentalized culture, the oil phase protects the small volume of culture medium from drying and increases the CO2 supply. Since a difference in cell growth was observed with and without the addition of antibiotics, this compartmentalized culture method could be a powerful tool for mutant selection.

AB - Photosynthetic microorganisms such as cyanobacteria have attracted attention for their potential to produce biofuels and biochemicals directly from CO2. Cell isolation by colony has conventionally been used for selecting target cells. Colony isolation methods require a significant amount of time for cultivation, and the colony-forming ratio is potentially low for cyanobacteria. Here, we overcome such limitations by encapsulating and culturing cells in droplets with an overlay of dodecane using glass slides printed with highly water-repellent mark. In the compartmentalized culture, the oil phase protects the small volume of culture medium from drying and increases the CO2 supply. Since a difference in cell growth was observed with and without the addition of antibiotics, this compartmentalized culture method could be a powerful tool for mutant selection.

UR - http://www.scopus.com/inward/record.url?scp=84926351084&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84926351084&partnerID=8YFLogxK

U2 - 10.1016/j.btre.2014.10.003

DO - 10.1016/j.btre.2014.10.003

M3 - Article

AN - SCOPUS:84926351084

VL - 4

SP - 151

EP - 155

JO - Biotechnology Reports

JF - Biotechnology Reports

SN - 2215-017X

ER -