Microanalysis of Nucleic Acids Using the Limulus G Test

Takahisa Anada; Kazuya Koumoto; Masami Mizu; Ryouji Karinaga; Kazuo Sakurai; Seiji Shinkai

doi:10.2116/analsci.19.1567

Microanalysis of Nucleic Acids Using the Limulus G Test

Takahisa Anada, Kazuya Koumoto, Masami Mizu, Ryouji Karinaga, Kazuo Sakurai, Seiji Shinkai

Institute for Advanced Study

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The limulus G test has been used as a quantitative analysis of (1→3)-β-D-glucans, including schizophyllan (SPG) and curdlan. The present work extended the limulus G test to detect polynucleotide/SPG complexes. The complex showed an extremely sensitive response to the test, compared with SPG itself. The minimum concentration of the complex to show the response is almost 10-times as small as that of SPG itself, indicating the possibility to detect (1→3)-β-D-glucans or/and polynucleotides on the pico gram/ml scale.

Original language	English
Pages (from-to)	1567-1568
Number of pages	2
Journal	analytical sciences
Volume	19
Issue number	12
DOIs	https://doi.org/10.2116/analsci.19.1567
Publication status	Published - Dec 2003

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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abstract = "The limulus G test has been used as a quantitative analysis of (1→3)-β-D-glucans, including schizophyllan (SPG) and curdlan. The present work extended the limulus G test to detect polynucleotide/SPG complexes. The complex showed an extremely sensitive response to the test, compared with SPG itself. The minimum concentration of the complex to show the response is almost 10-times as small as that of SPG itself, indicating the possibility to detect (1→3)-β-D-glucans or/and polynucleotides on the pico gram/ml scale.",

author = "Takahisa Anada and Kazuya Koumoto and Masami Mizu and Ryouji Karinaga and Kazuo Sakurai and Seiji Shinkai",

year = "2003",

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AU - Anada, Takahisa

AU - Koumoto, Kazuya

AU - Mizu, Masami

AU - Karinaga, Ryouji

AU - Sakurai, Kazuo

AU - Shinkai, Seiji

PY - 2003/12

Y1 - 2003/12

N2 - The limulus G test has been used as a quantitative analysis of (1→3)-β-D-glucans, including schizophyllan (SPG) and curdlan. The present work extended the limulus G test to detect polynucleotide/SPG complexes. The complex showed an extremely sensitive response to the test, compared with SPG itself. The minimum concentration of the complex to show the response is almost 10-times as small as that of SPG itself, indicating the possibility to detect (1→3)-β-D-glucans or/and polynucleotides on the pico gram/ml scale.

AB - The limulus G test has been used as a quantitative analysis of (1→3)-β-D-glucans, including schizophyllan (SPG) and curdlan. The present work extended the limulus G test to detect polynucleotide/SPG complexes. The complex showed an extremely sensitive response to the test, compared with SPG itself. The minimum concentration of the complex to show the response is almost 10-times as small as that of SPG itself, indicating the possibility to detect (1→3)-β-D-glucans or/and polynucleotides on the pico gram/ml scale.

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Microanalysis of Nucleic Acids Using the Limulus G Test

Abstract

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