Microanalysis of Nucleic Acids Using the Limulus G Test

Takahisa Anada, Kazuya Koumoto, Masami Mizu, Ryouji Karinaga, Kazuo Sakurai, Seiji Shinkai

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The limulus G test has been used as a quantitative analysis of (1→3)-β-D-glucans, including schizophyllan (SPG) and curdlan. The present work extended the limulus G test to detect polynucleotide/SPG complexes. The complex showed an extremely sensitive response to the test, compared with SPG itself. The minimum concentration of the complex to show the response is almost 10-times as small as that of SPG itself, indicating the possibility to detect (1→3)-β-D-glucans or/and polynucleotides on the pico gram/ml scale.

Original languageEnglish
Pages (from-to)1567-1568
Number of pages2
Journalanalytical sciences
Volume19
Issue number12
DOIs
Publication statusPublished - Dec 2003

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Microanalysis of Nucleic Acids Using the Limulus G Test'. Together they form a unique fingerprint.

  • Cite this

    Anada, T., Koumoto, K., Mizu, M., Karinaga, R., Sakurai, K., & Shinkai, S. (2003). Microanalysis of Nucleic Acids Using the Limulus G Test. analytical sciences, 19(12), 1567-1568. https://doi.org/10.2116/analsci.19.1567