Microarray analysis of Arabidopsis plants in response to allelochemical L-DOPA

Anna Golisz, Mami Sugano, Syuntaro Hiradate, Yoshiharu Fujii

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Velvetbean (Mucuna pruriens) plants impede the growth of neighboring plants. One compound, 3-(3′,4′-dihydroxyphenyl)-l-alanine (l-DOPA), is responsible for the allelopathic capacity of velvetbean. This compound is an active allelochemical that decreases root growth of several plant species. In mammals, l-DOPA is a well-known therapeutic agent for the symptomatic relief of Parkinson's disease. However, its mode of action in plants is still not well understood. To address such issues, gene expression in Arabidopsis thaliana plants, which had been exposed to l-DOPA, was analyzed using DNA microarrays. After 6 h of l-DOPA exposure, the expression of 110 genes was significantly upregulated, and the expression of 69 genes was significantly downregulated. These induced genes can be divided into different functional categories, mainly on the basis of subcellular localization, metabolism, and proteins with a binding function or cofactor requirement. Based on these results, we suggest that l-DOPA acts by two mechanisms: it influences amino acid metabolism and deregulates metal homeostasis, especially that of iron, which is required for the fundamental biological processes of all organisms.

Original languageEnglish
Pages (from-to)231-240
Number of pages10
JournalPlanta
Volume233
Issue number2
DOIs
Publication statusPublished - Jan 1 2011
Externally publishedYes

Fingerprint

Pheromones
allelochemicals
Microarray Analysis
Mucuna pruriens
Arabidopsis
plant response
Gene Expression
gene expression
Secondary Parkinson Disease
Mucuna
Parkinson disease
amino acid metabolism
Biological Phenomena
alanine
mechanism of action
root growth
homeostasis
Growth
Oligonucleotide Array Sequence Analysis
genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Cite this

Microarray analysis of Arabidopsis plants in response to allelochemical L-DOPA. / Golisz, Anna; Sugano, Mami; Hiradate, Syuntaro; Fujii, Yoshiharu.

In: Planta, Vol. 233, No. 2, 01.01.2011, p. 231-240.

Research output: Contribution to journalArticle

Golisz, Anna ; Sugano, Mami ; Hiradate, Syuntaro ; Fujii, Yoshiharu. / Microarray analysis of Arabidopsis plants in response to allelochemical L-DOPA. In: Planta. 2011 ; Vol. 233, No. 2. pp. 231-240.
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