Microarray analysis of gene expression in the diacylglycerol kinase η knockout mouse brain

Suguru Komenoi, Yuji Suzuki, Maho Asami, Chiaki Murakami, Fumi Hoshino, Sohei Chiba, Daisuke Takahashi, Sayaka Kado, Fumio Sakane

Research output: Contribution to journalArticle

Abstract

We have revealed that diacylglycerol kinase η (DGKη)-knockout (KO) mice display bipolar disorder (BPD) remedy-sensitive mania-like behaviors. However, the molecular mechanisms causing the mania-like abnormal behaviors remain unclear. In the present study, microarray analysis was performed to determine global changes in gene expression in the DGKη-KO mouse brain. We found that the DGKη-KO brain had 43 differentially expressed genes and the following five affected biological pathways: “neuroactive ligand-receptor interaction”, “transcription by RNA polymerase II”, “cytosolic calcium ion concentration”, “Jak-STAT signaling pathway” and “ERK1/2 cascade”. Interestingly, mRNA levels of prolactin and growth hormone, which are augmented in BPD patients and model animals, were most strongly increased. Notably, all five biological pathways include at least one gene among prolactin, growth hormone, forkhead box P3, glucagon-like peptide 1 receptor and interleukin 1β, which were previously implicated in BPD. Consistent with the microarray data, phosphorylated ERK1/2 levels were decreased in the DGKη-KO brain. Microarray analysis showed that the expression levels of several glycerolipid metabolism-related genes were also changed. Liquid chromatography-mass spectrometry revealed that several polyunsaturated fatty acid (PUFA)-containing phosphatidic acid (PA) molecular species were significantly decreased as a result of DGKη deficiency, suggesting that the decrease affects PUFA metabolism. Intriguingly, the PUFA-containing lysoPA species were markedly decreased in DGKη-KO mouse blood. Taken together, our study provides not only key broad knowledge to gain novel insights into the underlying mechanisms for the mania-like behaviors but also information for developing BPD diagnostics.

Original languageEnglish
Article number100660
JournalBiochemistry and Biophysics Reports
Volume19
DOIs
Publication statusPublished - Sep 1 2019

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Diacylglycerol Kinase
Microarray Analysis
Microarrays
Bipolar Disorder
Gene expression
Knockout Mice
Brain
Gene Expression
Unsaturated Fatty Acids
Genes
Metabolism
Prolactin
Growth Hormone
Phosphatidic Acids
RNA Polymerase II
Liquid chromatography
Transcription
MAP Kinase Signaling System
Interleukin-1
Mass spectrometry

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Komenoi, S., Suzuki, Y., Asami, M., Murakami, C., Hoshino, F., Chiba, S., ... Sakane, F. (2019). Microarray analysis of gene expression in the diacylglycerol kinase η knockout mouse brain. Biochemistry and Biophysics Reports, 19, [100660]. https://doi.org/10.1016/j.bbrep.2019.100660

Microarray analysis of gene expression in the diacylglycerol kinase η knockout mouse brain. / Komenoi, Suguru; Suzuki, Yuji; Asami, Maho; Murakami, Chiaki; Hoshino, Fumi; Chiba, Sohei; Takahashi, Daisuke; Kado, Sayaka; Sakane, Fumio.

In: Biochemistry and Biophysics Reports, Vol. 19, 100660, 01.09.2019.

Research output: Contribution to journalArticle

Komenoi, Suguru ; Suzuki, Yuji ; Asami, Maho ; Murakami, Chiaki ; Hoshino, Fumi ; Chiba, Sohei ; Takahashi, Daisuke ; Kado, Sayaka ; Sakane, Fumio. / Microarray analysis of gene expression in the diacylglycerol kinase η knockout mouse brain. In: Biochemistry and Biophysics Reports. 2019 ; Vol. 19.
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