Microarray technologies for intracellular kinome analysis

T. Yamamoto, T. Mori, Y. Katayama

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Microarray-based kinomics, which measure the enzymatic activity or the presence of intracellular protein kinases, are now regarded as alternative tools to conventional mass spectrometry-based kinomics for examining intracellular kinomics. Here, we reviewed the principal advantages, recent progress, and remaining problems of representative microarray- based kinomics, including substrate peptide and protein microarrays, anti-protein kinase antibody microarrays, and reverse protein microarrays. Microarray-based kinomics are not as good at quantitative evaluation of kinomics as the conventional mass spectrometry-based kinomics. However, their simplicity and high throughput make the microarraybased kinomics unique tools, being especially suited for a practical analysis; monitoring drug effects on cellular kinomics as a tool for drug development, and for the diagnosis and prognosis of diseases based on kinomics.

Original languageEnglish
Pages (from-to)2542-2552
Number of pages11
JournalCurrent Medicinal Chemistry
Volume21
Issue number22
DOIs
Publication statusPublished - 2014

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

Cite this