Microbial recovery of vanadium by the acidophilic bacterium, Acidocella aromatica

Naoko Okibe, Masashi Maki, Daisuke Nakayama, Keiko Sasaki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: To investigate the bioreduction of toxic pentavalent vanadium [vanadate; V(V)] in the acidophilic, Fe(III)-reducing obligately heterotrophic bacterium, Acidocella aromatica PFBC. Results: Although the initial lag-phase of growth became extended with increasing initial V(V) concentrations, the final cell density during aerobic growth of A. aromatica PFBC was unaffected by up to 2 mM V(V). While strain PFBC is an aerobe, growth-decoupled PFBC cell suspensions directly reduced V(V) using fructose, both micro-aerobically and anaerobically, under highly acidic (pH 2) and moderately acidic (pH 4.5) conditions. Bio reduced V(IV) was subsequently precipitated even under micro-aerobic conditions, mostly by encrusting the cell surface. An anaerobic condition at pH 4.5 was optimal for forming and maintaining stable V(IV)-precipitates. Recovery of approx. 70 % of V(V) from the solution phase was made possible with V(V) at 1 mM. Conclusions: The first case of direct V(V) reducing ability and its subsequent V recovery from the solution phase was shown in acidophilic prokaryotes. Possible utilities of V(V) bioreduction in acidic conditions, are discussed.

Original languageEnglish
Pages (from-to)1475-1481
Number of pages7
JournalBiotechnology letters
Volume38
Issue number9
DOIs
Publication statusPublished - Sep 1 2016

Fingerprint

Vanadium
Bacteria
Recovery
Growth
Fructose
Vanadates
Poisons
Precipitates
Suspensions
Cell Count

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Microbial recovery of vanadium by the acidophilic bacterium, Acidocella aromatica. / Okibe, Naoko; Maki, Masashi; Nakayama, Daisuke; Sasaki, Keiko.

In: Biotechnology letters, Vol. 38, No. 9, 01.09.2016, p. 1475-1481.

Research output: Contribution to journalArticle

@article{54615572dd4c46e9b5ff6a7dc1b40bca,
title = "Microbial recovery of vanadium by the acidophilic bacterium, Acidocella aromatica",
abstract = "Objective: To investigate the bioreduction of toxic pentavalent vanadium [vanadate; V(V)] in the acidophilic, Fe(III)-reducing obligately heterotrophic bacterium, Acidocella aromatica PFBC. Results: Although the initial lag-phase of growth became extended with increasing initial V(V) concentrations, the final cell density during aerobic growth of A. aromatica PFBC was unaffected by up to 2 mM V(V). While strain PFBC is an aerobe, growth-decoupled PFBC cell suspensions directly reduced V(V) using fructose, both micro-aerobically and anaerobically, under highly acidic (pH 2) and moderately acidic (pH 4.5) conditions. Bio reduced V(IV) was subsequently precipitated even under micro-aerobic conditions, mostly by encrusting the cell surface. An anaerobic condition at pH 4.5 was optimal for forming and maintaining stable V(IV)-precipitates. Recovery of approx. 70 {\%} of V(V) from the solution phase was made possible with V(V) at 1 mM. Conclusions: The first case of direct V(V) reducing ability and its subsequent V recovery from the solution phase was shown in acidophilic prokaryotes. Possible utilities of V(V) bioreduction in acidic conditions, are discussed.",
author = "Naoko Okibe and Masashi Maki and Daisuke Nakayama and Keiko Sasaki",
year = "2016",
month = "9",
day = "1",
doi = "10.1007/s10529-016-2131-2",
language = "English",
volume = "38",
pages = "1475--1481",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "9",

}

TY - JOUR

T1 - Microbial recovery of vanadium by the acidophilic bacterium, Acidocella aromatica

AU - Okibe, Naoko

AU - Maki, Masashi

AU - Nakayama, Daisuke

AU - Sasaki, Keiko

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Objective: To investigate the bioreduction of toxic pentavalent vanadium [vanadate; V(V)] in the acidophilic, Fe(III)-reducing obligately heterotrophic bacterium, Acidocella aromatica PFBC. Results: Although the initial lag-phase of growth became extended with increasing initial V(V) concentrations, the final cell density during aerobic growth of A. aromatica PFBC was unaffected by up to 2 mM V(V). While strain PFBC is an aerobe, growth-decoupled PFBC cell suspensions directly reduced V(V) using fructose, both micro-aerobically and anaerobically, under highly acidic (pH 2) and moderately acidic (pH 4.5) conditions. Bio reduced V(IV) was subsequently precipitated even under micro-aerobic conditions, mostly by encrusting the cell surface. An anaerobic condition at pH 4.5 was optimal for forming and maintaining stable V(IV)-precipitates. Recovery of approx. 70 % of V(V) from the solution phase was made possible with V(V) at 1 mM. Conclusions: The first case of direct V(V) reducing ability and its subsequent V recovery from the solution phase was shown in acidophilic prokaryotes. Possible utilities of V(V) bioreduction in acidic conditions, are discussed.

AB - Objective: To investigate the bioreduction of toxic pentavalent vanadium [vanadate; V(V)] in the acidophilic, Fe(III)-reducing obligately heterotrophic bacterium, Acidocella aromatica PFBC. Results: Although the initial lag-phase of growth became extended with increasing initial V(V) concentrations, the final cell density during aerobic growth of A. aromatica PFBC was unaffected by up to 2 mM V(V). While strain PFBC is an aerobe, growth-decoupled PFBC cell suspensions directly reduced V(V) using fructose, both micro-aerobically and anaerobically, under highly acidic (pH 2) and moderately acidic (pH 4.5) conditions. Bio reduced V(IV) was subsequently precipitated even under micro-aerobic conditions, mostly by encrusting the cell surface. An anaerobic condition at pH 4.5 was optimal for forming and maintaining stable V(IV)-precipitates. Recovery of approx. 70 % of V(V) from the solution phase was made possible with V(V) at 1 mM. Conclusions: The first case of direct V(V) reducing ability and its subsequent V recovery from the solution phase was shown in acidophilic prokaryotes. Possible utilities of V(V) bioreduction in acidic conditions, are discussed.

UR - http://www.scopus.com/inward/record.url?scp=84970015949&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84970015949&partnerID=8YFLogxK

U2 - 10.1007/s10529-016-2131-2

DO - 10.1007/s10529-016-2131-2

M3 - Article

C2 - 27230938

AN - SCOPUS:84970015949

VL - 38

SP - 1475

EP - 1481

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 9

ER -