Microchip analysis of plant glucosinolates

Maged Fouad, Mohammad Jabasini, Noritada Kaji, Kazuyoshi Terasaka, Manabu Tokeshi, Hajime Mizukami, Yoshinobu Baba

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We describe a new and selective analytical method for the separation and quantitation of plant glucosinolates. The new method, which utilizes microchip CE (μ-CE) with fluorescence detection, circumvents the multistep procedures characteristic of conventional methods. Glucosinolates form charge transfer complexes with the xanthene dyes phloxine-B and eosin-B. The glucosinolates-phloxine-B complex cannot be excited at 470 nm. Thus, the decrease in peak intensity of phloxine-B after complex formation is used to quantitatively measure total glucosinolates in Arabidopsis thaliana seeds. For qualitative analysis, complex formation with eosin-B is used. The sensitivity of eosin-B detection at excitation/emission 470 nm/540 nm was low. However, sensitivity increased following complex formation with sinigrin (≥3 μg/mL). A batch-learning, self-organizing map was applied to visualize and organize analytical data into 2-D matrix with similar and related data clustered together or near each other. This organized matrix was used to optimize electrophoretic conditions for the analysis. This study suggests potential applications of μ-CE in plant metabolomics analyses without use of labeling fluorophores.

Original languageEnglish
Pages (from-to)2280-2287
Number of pages8
JournalELECTROPHORESIS
Volume29
Issue number11
DOIs
Publication statusPublished - Jun 1 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

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