Microchip-based homogeneous immunoassay using a cloned enzyme donor

Tomoya Tachi, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme β-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 μg mL -1 . Our CEDIA using a microfluidic device was very simple and rapid, unlike microchipbased heterogeneous immunoassays and CEDIA on a well-type microchip.

Original languageEnglish
Pages (from-to)149-151
Number of pages3
Journalanalytical sciences
Volume25
Issue number2
DOIs
Publication statusPublished - 2009
Externally publishedYes

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Enzymes
Galactosidases
Theophylline
Microfluidics

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Microchip-based homogeneous immunoassay using a cloned enzyme donor. / Tachi, Tomoya; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

In: analytical sciences, Vol. 25, No. 2, 2009, p. 149-151.

Research output: Contribution to journalArticle

Tachi, Tomoya ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Microchip-based homogeneous immunoassay using a cloned enzyme donor. In: analytical sciences. 2009 ; Vol. 25, No. 2. pp. 149-151.
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