Abstract
A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (n = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.
| Original language | English |
|---|---|
| Pages (from-to) | 396-397 |
| Number of pages | 2 |
| Journal | Chemistry Letters |
| Volume | 36 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 5 2007 |
| Externally published | Yes |
Fingerprint
All Science Journal Classification (ASJC) codes
- Chemistry(all)
Cite this
Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis. / Mahmoudian, Laili; Melin, Jonas; Mohamadi, Mohamad Reza; Yamada, Keiko; Ohta, Michio; Kaji, Noritada; Tokeshi, Manabu; Nilsson, Mats; Baba, Yoshinobu.
In: Chemistry Letters, Vol. 36, No. 3, 05.03.2007, p. 396-397.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis
AU - Mahmoudian, Laili
AU - Melin, Jonas
AU - Mohamadi, Mohamad Reza
AU - Yamada, Keiko
AU - Ohta, Michio
AU - Kaji, Noritada
AU - Tokeshi, Manabu
AU - Nilsson, Mats
AU - Baba, Yoshinobu
PY - 2007/3/5
Y1 - 2007/3/5
N2 - A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (n = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.
AB - A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (n = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.
UR - http://www.scopus.com/inward/record.url?scp=34047271112&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34047271112&partnerID=8YFLogxK
U2 - 10.1246/cl.2007.396
DO - 10.1246/cl.2007.396
M3 - Article
AN - SCOPUS:34047271112
VL - 36
SP - 396
EP - 397
JO - Chemistry Letters
JF - Chemistry Letters
SN - 0366-7022
IS - 3
ER -