Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis

Laili Mahmoudian; Jonas Melin; Mohamad Reza Mohamadi; Keiko Yamada; Michio Ohta; Noritada Kaji; Manabu Tokeshi; Mats Nilsson; Yoshinobu Baba

doi:10.1246/cl.2007.396

Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis

Laili Mahmoudian, Jonas Melin, Mohamad Reza Mohamadi, Keiko Yamada, Michio Ohta, Noritada Kaji, Manabu Tokeshi, Mats Nilsson, Yoshinobu Baba

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (n = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.

Original language	English
Pages (from-to)	396-397
Number of pages	2
Journal	Chemistry Letters
Volume	36
Issue number	3
DOIs	https://doi.org/10.1246/cl.2007.396
Publication status	Published - Mar 5 2007
Externally published	Yes

All Science Journal Classification (ASJC) codes

Chemistry(all)

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10.1246/cl.2007.396

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title = "Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis",

abstract = "A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (n = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.",

author = "Laili Mahmoudian and Jonas Melin and Mohamadi, {Mohamad Reza} and Keiko Yamada and Michio Ohta and Noritada Kaji and Manabu Tokeshi and Mats Nilsson and Yoshinobu Baba",

year = "2007",

month = mar,

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doi = "10.1246/cl.2007.396",

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volume = "36",

pages = "396--397",

journal = "Chemistry Letters",

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publisher = "The Chemical Society of Japan",

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T1 - Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis

AU - Mahmoudian, Laili

AU - Melin, Jonas

AU - Mohamadi, Mohamad Reza

AU - Yamada, Keiko

AU - Ohta, Michio

AU - Kaji, Noritada

AU - Tokeshi, Manabu

AU - Nilsson, Mats

AU - Baba, Yoshinobu

PY - 2007/3/5

Y1 - 2007/3/5

N2 - A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (n = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.

AB - A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (n = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.

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EP - 397

JO - Chemistry Letters

JF - Chemistry Letters

SN - 0366-7022

IS - 3

ER -

Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis

Abstract

All Science Journal Classification (ASJC) codes

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