Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis

Laili Mahmoudian, Jonas Melin, Mohamad Reza Mohamadi, Keiko Yamada, Michio Ohta, Noritada Kaji, Manabu Tokeshi, Mats Nilsson, Yoshinobu Baba

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (n = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.

Original languageEnglish
Pages (from-to)396-397
Number of pages2
JournalChemistry Letters
Volume36
Issue number3
DOIs
Publication statusPublished - Mar 5 2007
Externally publishedYes

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Pathogens
Electrophoresis
Amplification
Nucleotides
DNA

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

Cite this

Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis. / Mahmoudian, Laili; Melin, Jonas; Mohamadi, Mohamad Reza; Yamada, Keiko; Ohta, Michio; Kaji, Noritada; Tokeshi, Manabu; Nilsson, Mats; Baba, Yoshinobu.

In: Chemistry Letters, Vol. 36, No. 3, 05.03.2007, p. 396-397.

Research output: Contribution to journalArticle

Mahmoudian, L, Melin, J, Mohamadi, MR, Yamada, K, Ohta, M, Kaji, N, Tokeshi, M, Nilsson, M & Baba, Y 2007, 'Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis', Chemistry Letters, vol. 36, no. 3, pp. 396-397. https://doi.org/10.1246/cl.2007.396
Mahmoudian, Laili ; Melin, Jonas ; Mohamadi, Mohamad Reza ; Yamada, Keiko ; Ohta, Michio ; Kaji, Noritada ; Tokeshi, Manabu ; Nilsson, Mats ; Baba, Yoshinobu. / Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis. In: Chemistry Letters. 2007 ; Vol. 36, No. 3. pp. 396-397.
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