Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis

Laili Mahmoudian; Jonas Melin; Mohamad Reza Mohamadi; Keiko Yamada; Michio Ohta; Noritada Kaji; Manabu Tokeshi; Mats Nilsson; Yoshinobu Baba

doi:10.1246/cl.2007.396

Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis

Laili Mahmoudian, Jonas Melin, Mohamad Reza Mohamadi, Keiko Yamada, Michio Ohta, Noritada Kaji, Manabu Tokeshi, Mats Nilsson, Yoshinobu Baba

Molecular Information Chemistry

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A new method for fast and precise analysis of circle-to-circle amplification (C2CA) products by microchip electrophoresis has been developed. Stable C2CA products were produced by applying a new enzymatic step to C2CA. Detection was carried out within 55s with RSD of migration time of 3.6% (n = 6) enabling reproducibility and high speed. A real sample of bacterial pathogen (V. Cholerae) at single nucleotide level was detected successfully based on this method.

Original language	English
Pages (from-to)	396-397
Number of pages	2
Journal	Chemistry Letters
Volume	36
Issue number	3
DOIs	https://doi.org/10.1246/cl.2007.396
Publication status	Published - Mar 5 2007

All Science Journal Classification (ASJC) codes

Chemistry(all)

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10.1246/cl.2007.396

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Microchip electrophoresis for detection of circle-to-circle amplification products towards sensitive and rapid DNA analysis. / Mahmoudian, Laili; Melin, Jonas; Mohamadi, Mohamad Reza; Yamada, Keiko; Ohta, Michio; Kaji, Noritada; Tokeshi, Manabu; Nilsson, Mats; Baba, Yoshinobu.

In: Chemistry Letters, Vol. 36, No. 3, 05.03.2007, p. 396-397.

Research output: Contribution to journal › Article › peer-review

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AU - Melin, Jonas

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AU - Yamada, Keiko

AU - Ohta, Michio

AU - Kaji, Noritada

AU - Tokeshi, Manabu

AU - Nilsson, Mats

AU - Baba, Yoshinobu

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