Microchip electrophoretic mobility shift assay for detecting interactions of DNA-transcription factor NFκB

Sonoko Inoue; Masatoshi Kataoka; Yasuo Shinohara; Noritada Kaji; Manabu Tokeshi; Yoshinobu Baba

Microchip electrophoretic mobility shift assay for detecting interactions of DNA-transcription factor NFκB

Sonoko Inoue, Masatoshi Kataoka, Yasuo Shinohara, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to conference › Paper › peer-review

Abstract

Microchip electrophoretic mobility shift assay (μEMSA) has been developed for DNA-protein interaction study, and finally, we succeed in detecting the interaction of DNA and Nuclear Factor κB (NFκB) within only 200 s. Since the method provides quantitative data simultaneously, kinetics data analysis of DNA-protein interaction was also conducted.

Original language	English
Pages	903-905
Number of pages	3
Publication status	Published - Jan 1 2006
Externally published	Yes
Event	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: Nov 5 2006 → Nov 9 2006

Other

Other	10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/Territory	Japan
City	Tokyo
Period	11/5/06 → 11/9/06

All Science Journal Classification (ASJC) codes

Chemical Engineering (miscellaneous)
Bioengineering

Cite this

Microchip electrophoretic mobility shift assay for detecting interactions of DNA-transcription factor NFκB. / Inoue, Sonoko; Kataoka, Masatoshi; Shinohara, Yasuo et al.

2006. 903-905 Paper presented at 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006, Tokyo, Japan.

Research output: Contribution to conference › Paper › peer-review

@conference{c3d897cdd55e4c25a5c4a51c9bc77931,

title = "Microchip electrophoretic mobility shift assay for detecting interactions of DNA-transcription factor NFκB",

abstract = "Microchip electrophoretic mobility shift assay (μEMSA) has been developed for DNA-protein interaction study, and finally, we succeed in detecting the interaction of DNA and Nuclear Factor κB (NFκB) within only 200 s. Since the method provides quantitative data simultaneously, kinetics data analysis of DNA-protein interaction was also conducted.",

author = "Sonoko Inoue and Masatoshi Kataoka and Yasuo Shinohara and Noritada Kaji and Manabu Tokeshi and Yoshinobu Baba",

year = "2006",

month = jan,

day = "1",

language = "English",

pages = "903--905",

note = "10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 ; Conference date: 05-11-2006 Through 09-11-2006",

}

TY - CONF

T1 - Microchip electrophoretic mobility shift assay for detecting interactions of DNA-transcription factor NFκB

AU - Inoue, Sonoko

AU - Kataoka, Masatoshi

AU - Shinohara, Yasuo

AU - Kaji, Noritada

AU - Tokeshi, Manabu

AU - Baba, Yoshinobu

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Microchip electrophoretic mobility shift assay (μEMSA) has been developed for DNA-protein interaction study, and finally, we succeed in detecting the interaction of DNA and Nuclear Factor κB (NFκB) within only 200 s. Since the method provides quantitative data simultaneously, kinetics data analysis of DNA-protein interaction was also conducted.

AB - Microchip electrophoretic mobility shift assay (μEMSA) has been developed for DNA-protein interaction study, and finally, we succeed in detecting the interaction of DNA and Nuclear Factor κB (NFκB) within only 200 s. Since the method provides quantitative data simultaneously, kinetics data analysis of DNA-protein interaction was also conducted.

UR - http://www.scopus.com/inward/record.url?scp=84902477636&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902477636&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902477636

SP - 903

EP - 905

T2 - 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006

Y2 - 5 November 2006 through 9 November 2006

ER -

Microchip electrophoretic mobility shift assay for detecting interactions of DNA-transcription factor NFκB

Abstract

Other

All Science Journal Classification (ASJC) codes

Other files and links

Fingerprint

Cite this