Microchip electrophoretic mobility shift assay for detecting interactions of DNA-transcription factor NFκB

Sonoko Inoue, Masatoshi Kataoka, Yasuo Shinohara, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to conferencePaperpeer-review

Abstract

Microchip electrophoretic mobility shift assay (μEMSA) has been developed for DNA-protein interaction study, and finally, we succeed in detecting the interaction of DNA and Nuclear Factor κB (NFκB) within only 200 s. Since the method provides quantitative data simultaneously, kinetics data analysis of DNA-protein interaction was also conducted.

Original languageEnglish
Pages903-905
Number of pages3
Publication statusPublished - Jan 1 2006
Externally publishedYes
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: Nov 5 2006Nov 9 2006

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
CountryJapan
CityTokyo
Period11/5/0611/9/06

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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