Microchip immunoassays for monitoring renal function: Rapid, low‐cost, and highly sensitive quantification of urinary biomarkers of diabetic nephropathy

Toshihiro Kasama, Miaomiao Sun, Noritada Kaji, Shin’Ichi Akiyama, Yukio Yuzawa, Manabu Tokeshi, Seiichi Matsuo, Yoshinobu Baba

Research output: Contribution to journalArticlepeer-review

Abstract

This study developed low‐cost and highly sensitive immunoassay devices possessing the ability to rapidly analyze urine samples. Further, they can quantitatively detect three biomarkers indicating renal injury: monocyte chemotactic protein 1 (MCP‐1), angiotensinogen (AGT), and liver-type fatty acid binding protein (L‐FABP). The devices were used to successfully estimate the concentrations of the three biomarkers in urine samples within 2 min; the results were consistent with those obtained via conventional enzyme‐linked immunosorbent assay (ELISA), which requires several hours. In addition, the estimated detection limits for the three biomarkers were comparable to those of commercially available ELISA kits. Thus, the proposed and fabricated devices facilitate high‐precision and frequent monitoring of renal function.

Original languageEnglish
Article number1353
JournalMicromachines
Volume12
Issue number11
DOIs
Publication statusPublished - Nov 2021

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Fingerprint

Dive into the research topics of 'Microchip immunoassays for monitoring renal function: Rapid, low‐cost, and highly sensitive quantification of urinary biomarkers of diabetic nephropathy'. Together they form a unique fingerprint.

Cite this