Microfluidic cell separation with antibody modified euglena by using phototaxis mediated migration

Yukihiro Okamoto, Yukinori Nakakita, Takahiro Sano, Jumpei Morikawa, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We develop a microfluidic cell separation method which combined microfluidic devices and multifunctional Euglena. Multifunctional Euglena for cell separation was successfully prepared by the surface modification of Euglena cell membrane and its performances were confirmed. Mutlifunctional Euglena in the inlet attached cells, migrated inside the microchannel by phototaxis towards the outlet chamber, and brought specific cells to the outlet. After moving to the outlet, cells in the outlet were detached from Euglena. Since it is based on the unique characters of Euglena, our method needs only simple device and can deal with small to large amount variety kinds of samples including cells.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1909-1911
Number of pages3
Publication statusPublished - Dec 1 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Fingerprint

Microfluidics
Antibodies
Cell membranes
Microchannels
Surface treatment

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Okamoto, Y., Nakakita, Y., Sano, T., Morikawa, J., Kaji, N., Tokeshi, M., & Baba, Y. (2011). Microfluidic cell separation with antibody modified euglena by using phototaxis mediated migration. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1909-1911). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).

Microfluidic cell separation with antibody modified euglena by using phototaxis mediated migration. / Okamoto, Yukihiro; Nakakita, Yukinori; Sano, Takahiro; Morikawa, Jumpei; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1909-1911 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Okamoto, Y, Nakakita, Y, Sano, T, Morikawa, J, Kaji, N, Tokeshi, M & Baba, Y 2011, Microfluidic cell separation with antibody modified euglena by using phototaxis mediated migration. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 3, pp. 1909-1911, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.
Okamoto Y, Nakakita Y, Sano T, Morikawa J, Kaji N, Tokeshi M et al. Microfluidic cell separation with antibody modified euglena by using phototaxis mediated migration. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 1909-1911. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
Okamoto, Yukihiro ; Nakakita, Yukinori ; Sano, Takahiro ; Morikawa, Jumpei ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Microfluidic cell separation with antibody modified euglena by using phototaxis mediated migration. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 1909-1911 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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