Microfluidic Chip for Simultaneous Loading and Injection of a Single Cell by Using Rack and Pinion Mechanisms

Kevin T. Uning, Keita Ichikawa, Akiho Hirao, Taiga Michimoto, Tasuku Sato, Hiroaki Kume, Takumi Nishida, Shota Iwakawa, Yoko Yamanishi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present a novel microinjection chip, which combines a pinion-rack cell loader and a 3-dimensional bubble injector probe, in order to increase the efficiency of microinjection and simplify it. The loader features a powerful interaction with magnets, capable of lifting as much as 20 grams with a 400mT strong magnet. Additionally, the bubble injector is easy to use while also being reliable in doing microinjections. Consequently, not only that the loader can work under harsh conditions, it does so while maintaining a high throughput of up to 16 gel beads (as the model of cells) per minutes. Combining this reliable loader with the easily operated injector, this chip will allow anyone with little to no training to perform microinjections.

Original languageEnglish
Title of host publication33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages392-395
Number of pages4
ISBN (Electronic)9781728135809
DOIs
Publication statusPublished - Jan 2020
Event33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 - Vancouver, Canada
Duration: Jan 18 2020Jan 22 2020

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2020-January
ISSN (Print)1084-6999

Conference

Conference33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020
CountryCanada
CityVancouver
Period1/18/201/22/20

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Uning, K. T., Ichikawa, K., Hirao, A., Michimoto, T., Sato, T., Kume, H., Nishida, T., Iwakawa, S., & Yamanishi, Y. (2020). Microfluidic Chip for Simultaneous Loading and Injection of a Single Cell by Using Rack and Pinion Mechanisms. In 33rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2020 (pp. 392-395). [9056117] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2020-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMS46641.2020.9056117