Microfluidic device for on-chip manipulation of liquid plugs for biosensing applications

Yoshifumi Shimizu, Wataru Satoh, Atsushi Takashima, Fumihiro Sassa, Junji Fukuda, Hiroaki Suzuki

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

A microfluidic device that forms a row of liquid plugs in a micro flow channel was fabricated, and its applicability to an on-chip enzyme-linked immunosorbent assay (ELISA) was demonstrated. The nanoliter liquid plugs were formed by six independent pumps and were mobilized in the micro-flow channel using a main pump. The operation of the pumps was based on the volume change caused as a result of the electrochemical production of hydrogen bubbles. When the bubbles were produced in the pumps, a polydimethylsiloxane (PDMS) diaphragm at the bottom of the compartment inflated and closed an inlet hole at the top of the reservoir. The solution in the reservoir was forced to be injected into the flow channel forming a liquid plug. After six plugs were formed, they were mobilized to a reaction chamber one by one to allow an antigen-antibody binding, cleansing, and detection. This simple and reliable liquid handling system could be applied to the detection of a tumor maker, α-fetoprotein (AFP).

Original languageEnglish
Title of host publicationThe 6th IEEE Conference on SENSORS, IEEE SENSORS 2007
Pages470-473
Number of pages4
DOIs
Publication statusPublished - Dec 1 2007
Externally publishedYes
Event6th IEEE Conference on SENSORS, IEEE SENSORS 2007 - Atlanta, GA, United States
Duration: Oct 28 2007Oct 31 2007

Other

Other6th IEEE Conference on SENSORS, IEEE SENSORS 2007
CountryUnited States
CityAtlanta, GA
Period10/28/0710/31/07

All Science Journal Classification (ASJC) codes

  • Engineering (miscellaneous)
  • Electrical and Electronic Engineering

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