Microfluidic device for on-chip manipulation of liquid plugs for biosensing applications

Yoshifumi Shimizu; Wataru Satoh; Atsushi Takashima; Fumihiro Sassa; Junji Fukuda; Hiroaki Suzuki

doi:10.1109/ICSENS.2007.4388438

Microfluidic device for on-chip manipulation of liquid plugs for biosensing applications

Yoshifumi Shimizu, Wataru Satoh, Atsushi Takashima, Fumihiro Sassa, Junji Fukuda, Hiroaki Suzuki

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

A microfluidic device that forms a row of liquid plugs in a micro flow channel was fabricated, and its applicability to an on-chip enzyme-linked immunosorbent assay (ELISA) was demonstrated. The nanoliter liquid plugs were formed by six independent pumps and were mobilized in the micro-flow channel using a main pump. The operation of the pumps was based on the volume change caused as a result of the electrochemical production of hydrogen bubbles. When the bubbles were produced in the pumps, a polydimethylsiloxane (PDMS) diaphragm at the bottom of the compartment inflated and closed an inlet hole at the top of the reservoir. The solution in the reservoir was forced to be injected into the flow channel forming a liquid plug. After six plugs were formed, they were mobilized to a reaction chamber one by one to allow an antigen-antibody binding, cleansing, and detection. This simple and reliable liquid handling system could be applied to the detection of a tumor maker, α-fetoprotein (AFP).

Original language	English
Title of host publication	The 6th IEEE Conference on SENSORS, IEEE SENSORS 2007
Pages	470-473
Number of pages	4
DOIs	https://doi.org/10.1109/ICSENS.2007.4388438
Publication status	Published - Dec 1 2007
Externally published	Yes
Event	6th IEEE Conference on SENSORS, IEEE SENSORS 2007 - Atlanta, GA, United States Duration: Oct 28 2007 → Oct 31 2007

Other

Other	6th IEEE Conference on SENSORS, IEEE SENSORS 2007
Country/Territory	United States
City	Atlanta, GA
Period	10/28/07 → 10/31/07

All Science Journal Classification (ASJC) codes

Engineering (miscellaneous)
Electrical and Electronic Engineering

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10.1109/ICSENS.2007.4388438

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Shimizu, Y, Satoh, W, Takashima, A, Sassa, F, Fukuda, J & Suzuki, H 2007, Microfluidic device for on-chip manipulation of liquid plugs for biosensing applications. in The 6th IEEE Conference on SENSORS, IEEE SENSORS 2007., 4388438, pp. 470-473, 6th IEEE Conference on SENSORS, IEEE SENSORS 2007, Atlanta, GA, United States, 10/28/07. https://doi.org/10.1109/ICSENS.2007.4388438

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title = "Microfluidic device for on-chip manipulation of liquid plugs for biosensing applications",

abstract = "A microfluidic device that forms a row of liquid plugs in a micro flow channel was fabricated, and its applicability to an on-chip enzyme-linked immunosorbent assay (ELISA) was demonstrated. The nanoliter liquid plugs were formed by six independent pumps and were mobilized in the micro-flow channel using a main pump. The operation of the pumps was based on the volume change caused as a result of the electrochemical production of hydrogen bubbles. When the bubbles were produced in the pumps, a polydimethylsiloxane (PDMS) diaphragm at the bottom of the compartment inflated and closed an inlet hole at the top of the reservoir. The solution in the reservoir was forced to be injected into the flow channel forming a liquid plug. After six plugs were formed, they were mobilized to a reaction chamber one by one to allow an antigen-antibody binding, cleansing, and detection. This simple and reliable liquid handling system could be applied to the detection of a tumor maker, α-fetoprotein (AFP).",

author = "Yoshifumi Shimizu and Wataru Satoh and Atsushi Takashima and Fumihiro Sassa and Junji Fukuda and Hiroaki Suzuki",

year = "2007",

month = dec,

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doi = "10.1109/ICSENS.2007.4388438",

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pages = "470--473",

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note = "6th IEEE Conference on SENSORS, IEEE SENSORS 2007 ; Conference date: 28-10-2007 Through 31-10-2007",

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T1 - Microfluidic device for on-chip manipulation of liquid plugs for biosensing applications

AU - Shimizu, Yoshifumi

AU - Satoh, Wataru

AU - Takashima, Atsushi

AU - Sassa, Fumihiro

AU - Fukuda, Junji

AU - Suzuki, Hiroaki

PY - 2007/12/1

Y1 - 2007/12/1

N2 - A microfluidic device that forms a row of liquid plugs in a micro flow channel was fabricated, and its applicability to an on-chip enzyme-linked immunosorbent assay (ELISA) was demonstrated. The nanoliter liquid plugs were formed by six independent pumps and were mobilized in the micro-flow channel using a main pump. The operation of the pumps was based on the volume change caused as a result of the electrochemical production of hydrogen bubbles. When the bubbles were produced in the pumps, a polydimethylsiloxane (PDMS) diaphragm at the bottom of the compartment inflated and closed an inlet hole at the top of the reservoir. The solution in the reservoir was forced to be injected into the flow channel forming a liquid plug. After six plugs were formed, they were mobilized to a reaction chamber one by one to allow an antigen-antibody binding, cleansing, and detection. This simple and reliable liquid handling system could be applied to the detection of a tumor maker, α-fetoprotein (AFP).

AB - A microfluidic device that forms a row of liquid plugs in a micro flow channel was fabricated, and its applicability to an on-chip enzyme-linked immunosorbent assay (ELISA) was demonstrated. The nanoliter liquid plugs were formed by six independent pumps and were mobilized in the micro-flow channel using a main pump. The operation of the pumps was based on the volume change caused as a result of the electrochemical production of hydrogen bubbles. When the bubbles were produced in the pumps, a polydimethylsiloxane (PDMS) diaphragm at the bottom of the compartment inflated and closed an inlet hole at the top of the reservoir. The solution in the reservoir was forced to be injected into the flow channel forming a liquid plug. After six plugs were formed, they were mobilized to a reaction chamber one by one to allow an antigen-antibody binding, cleansing, and detection. This simple and reliable liquid handling system could be applied to the detection of a tumor maker, α-fetoprotein (AFP).

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DO - 10.1109/ICSENS.2007.4388438

M3 - Conference contribution

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SN - 1424412617

SN - 9781424412617

SP - 470

EP - 473

BT - The 6th IEEE Conference on SENSORS, IEEE SENSORS 2007

T2 - 6th IEEE Conference on SENSORS, IEEE SENSORS 2007

Y2 - 28 October 2007 through 31 October 2007

ER -

Microfluidic device for on-chip manipulation of liquid plugs for biosensing applications

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