Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection

Miho Tsuwaki, Jun Mizuno, Takashi Kasahara, Tomohiko Edura, Eri Kunisawa, Ryoichi Ishimatsu, Shigeyuki Matsunami, Toshihiko Imato, Chihaya Adachi, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

We propose a portable electrochemiluminescence (ECL)-induced fluorescence chip which consists of flow channels for fluorescence sample and multi-color emitting ECL excitation source. A prototype ECL-induced fluorescence chip was fabricated by conventional photolithography and bonding technique. Device performance was evaluated using ECL of rubrene as excitation source and resorufin as fluorescent dye. Fluorescence of 500 μM resorufin (600 nm) was successfully detected using 10 mM rubrene solution (560 nm) under the applied voltage of 4 V. The proposed principle is applicable for portable and on-demand multi fluorescence detection device using its freedom of choice for combination of the ECL light source.

Original languageEnglish
Title of host publicationMEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages108-111
Number of pages4
ISBN (Print)9781479935086
DOIs
Publication statusPublished - 2014
Event27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014 - San Francisco, CA, United States
Duration: Jan 26 2014Jan 30 2014

Other

Other27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014
CountryUnited States
CitySan Francisco, CA
Period1/26/141/30/14

Fingerprint

Microfluidics
Fluorescence
fluorescence
cells
chips
Photolithography
channel flow
Channel flow
photolithography
Fluorescent Dyes
excitation
Light sources
light sources
Dyes
dyes
prototypes
Color
color
Electric potential
electric potential

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Tsuwaki, M., Mizuno, J., Kasahara, T., Edura, T., Kunisawa, E., Ishimatsu, R., ... Shoji, S. (2014). Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems (pp. 108-111). [6765585] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2014.6765585

Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. / Tsuwaki, Miho; Mizuno, Jun; Kasahara, Takashi; Edura, Tomohiko; Kunisawa, Eri; Ishimatsu, Ryoichi; Matsunami, Shigeyuki; Imato, Toshihiko; Adachi, Chihaya; Shoji, Shuichi.

MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2014. p. 108-111 6765585.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Tsuwaki, M, Mizuno, J, Kasahara, T, Edura, T, Kunisawa, E, Ishimatsu, R, Matsunami, S, Imato, T, Adachi, C & Shoji, S 2014, Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. in MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems., 6765585, Institute of Electrical and Electronics Engineers Inc., pp. 108-111, 27th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2014, San Francisco, CA, United States, 1/26/14. https://doi.org/10.1109/MEMSYS.2014.6765585
Tsuwaki M, Mizuno J, Kasahara T, Edura T, Kunisawa E, Ishimatsu R et al. Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. In MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc. 2014. p. 108-111. 6765585 https://doi.org/10.1109/MEMSYS.2014.6765585
Tsuwaki, Miho ; Mizuno, Jun ; Kasahara, Takashi ; Edura, Tomohiko ; Kunisawa, Eri ; Ishimatsu, Ryoichi ; Matsunami, Shigeyuki ; Imato, Toshihiko ; Adachi, Chihaya ; Shoji, Shuichi. / Microfluidic electrochemiluminescence (ECL) integrated flow cell for portable fluorescence detection. MEMS 2014 - 27th IEEE International Conference on Micro Electro Mechanical Systems. Institute of Electrical and Electronics Engineers Inc., 2014. pp. 108-111
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