Microfluidic Mechanotyping of a Single Cell with Two Consecutive Constrictions of Different Sizes and an Electrical Detection System

Mamiko Sano, Noritada Kaji, Amy C. Rowat, Hirotoshi Yasaki, Long Shao, Hidefumi Odaka, Takao Yasui, Tetsuya Higashiyama, Yoshinobu Baba

Research output: Contribution to journalArticle

Abstract

The mechanical properties of a cell, which include parameters such as elasticity, inner pressure, and tensile strength, are extremely important because changes in these properties are indicative of diseases ranging from diabetes to malignant transformation. Considering the heterogeneity within a population of cancer cells, a robust measurement system at the single cell level is required for research and in clinical purposes. In this study, a potential microfluidic device for high-throughput and practical mechanotyping were developed to investigate the deformability and sizes of cells through a single run. This mechanotyping device consisted of two different sizes of consecutive constrictions in a microchannel and measured the size of cells and related deformability during transit. Cell deformability was evaluated based on the transit and on the effects of cytoskeleton-affecting drugs, which were detected within 50 ms. The mechanotyping device was able to also measure a cell cycle without the use of fluorescent or protein tags.

Original languageEnglish
Pages (from-to)12890-12899
Number of pages10
JournalAnalytical chemistry
Volume91
Issue number20
DOIs
Publication statusPublished - Oct 15 2019

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Formability
Microfluidics
Cells
Medical problems
Microchannels
Elasticity
Tensile strength
Throughput
Mechanical properties
Pharmaceutical Preparations
Proteins

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Microfluidic Mechanotyping of a Single Cell with Two Consecutive Constrictions of Different Sizes and an Electrical Detection System. / Sano, Mamiko; Kaji, Noritada; Rowat, Amy C.; Yasaki, Hirotoshi; Shao, Long; Odaka, Hidefumi; Yasui, Takao; Higashiyama, Tetsuya; Baba, Yoshinobu.

In: Analytical chemistry, Vol. 91, No. 20, 15.10.2019, p. 12890-12899.

Research output: Contribution to journalArticle

Sano, Mamiko ; Kaji, Noritada ; Rowat, Amy C. ; Yasaki, Hirotoshi ; Shao, Long ; Odaka, Hidefumi ; Yasui, Takao ; Higashiyama, Tetsuya ; Baba, Yoshinobu. / Microfluidic Mechanotyping of a Single Cell with Two Consecutive Constrictions of Different Sizes and an Electrical Detection System. In: Analytical chemistry. 2019 ; Vol. 91, No. 20. pp. 12890-12899.
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