TY - JOUR
T1 - Microfluidic Mechanotyping of a Single Cell with Two Consecutive Constrictions of Different Sizes and an Electrical Detection System
AU - Sano, Mamiko
AU - Kaji, Noritada
AU - Rowat, Amy C.
AU - Yasaki, Hirotoshi
AU - Shao, Long
AU - Odaka, Hidefumi
AU - Yasui, Takao
AU - Higashiyama, Tetsuya
AU - Baba, Yoshinobu
N1 - Funding Information:
This work was supported by Japan Science and Technology Agency (JST), PRESTO (No. JPMJPR16F4, No. JPMJPR151B), the Center of Innovation Program from JST, Nanotechnology Platform Program (Molecule and Material Synthesis) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan, a JSPS Grant-in-Aid for Scientific Research (A) 24241050, and the research grant from The Nitto Foundation. N.K. would like to express thanks to Ms. Yoko Suzuki and Ms. Hikaru Nakamura for their technical assistance.
Publisher Copyright:
Copyright © 2019 American Chemical Society.
PY - 2019/10/15
Y1 - 2019/10/15
N2 - The mechanical properties of a cell, which include parameters such as elasticity, inner pressure, and tensile strength, are extremely important because changes in these properties are indicative of diseases ranging from diabetes to malignant transformation. Considering the heterogeneity within a population of cancer cells, a robust measurement system at the single cell level is required for research and in clinical purposes. In this study, a potential microfluidic device for high-throughput and practical mechanotyping were developed to investigate the deformability and sizes of cells through a single run. This mechanotyping device consisted of two different sizes of consecutive constrictions in a microchannel and measured the size of cells and related deformability during transit. Cell deformability was evaluated based on the transit and on the effects of cytoskeleton-affecting drugs, which were detected within 50 ms. The mechanotyping device was able to also measure a cell cycle without the use of fluorescent or protein tags.
AB - The mechanical properties of a cell, which include parameters such as elasticity, inner pressure, and tensile strength, are extremely important because changes in these properties are indicative of diseases ranging from diabetes to malignant transformation. Considering the heterogeneity within a population of cancer cells, a robust measurement system at the single cell level is required for research and in clinical purposes. In this study, a potential microfluidic device for high-throughput and practical mechanotyping were developed to investigate the deformability and sizes of cells through a single run. This mechanotyping device consisted of two different sizes of consecutive constrictions in a microchannel and measured the size of cells and related deformability during transit. Cell deformability was evaluated based on the transit and on the effects of cytoskeleton-affecting drugs, which were detected within 50 ms. The mechanotyping device was able to also measure a cell cycle without the use of fluorescent or protein tags.
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U2 - 10.1021/acs.analchem.9b02818
DO - 10.1021/acs.analchem.9b02818
M3 - Article
C2 - 31442026
AN - SCOPUS:85073182685
VL - 91
SP - 12890
EP - 12899
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 20
ER -
