Microfluidic rare cancer cell collection with anti-EpCAM antibody modified euglena by phototaxis inside microchannels

Yukihiro Okamoto, Yukinori Nakakita, Takahiro Sano, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We develop a microfluidic cell separation method which combined microfluidic devices and multifunctional Euglena. Multifunctional Euglena for cell separation was successfully prepared by the surface modification of Euglena cell membrane and its performances were confirmed. Mutlifunctional Euglena in the inlet attached cells, migrated inside the microchannel by phototaxis towards the outlet chamber, and brought specific cells to the outlet. After moving to the outlet, cells in the outlet were detached from Euglena. Since it is based on the unique characters of Euglena, our method needs only simple device and can deal with small to large amount variety kinds of samples including cells.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1108-1110
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - Jan 1 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Okamoto, Y., Nakakita, Y., Sano, T., Kaji, N., Tokeshi, M., & Baba, Y. (2012). Microfluidic rare cancer cell collection with anti-EpCAM antibody modified euglena by phototaxis inside microchannels. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1108-1110). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.