Microfluidic single-molecule nuclease digestion reveals rate-enhancing off-and-on molecular encountering function for site-specific DNA break

D. Onoshima, Noritada Kaji, M. Tokeshi, Y. Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

An intermittent molecular encounter leading to a site-specific DNA-break was unveiled under a limited protein's sliding-free condition. It reflects a transient molecular action by which the reaction rate and efficiency of restriction enzyme in bacterial cells is enhanced. This was experimentally verified for the first time in the world by using our microfluidic device.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages820-822
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - Jan 1 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

Fingerprint

Microfluidics
Reaction rates
DNA
Enzymes
Proteins
Molecules

All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Onoshima, D., Kaji, N., Tokeshi, M., & Baba, Y. (2013). Microfluidic single-molecule nuclease digestion reveals rate-enhancing off-and-on molecular encountering function for site-specific DNA break. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 820-822). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.

Microfluidic single-molecule nuclease digestion reveals rate-enhancing off-and-on molecular encountering function for site-specific DNA break. / Onoshima, D.; Kaji, Noritada; Tokeshi, M.; Baba, Y.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. p. 820-822 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Onoshima, D, Kaji, N, Tokeshi, M & Baba, Y 2013, Microfluidic single-molecule nuclease digestion reveals rate-enhancing off-and-on molecular encountering function for site-specific DNA break. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, vol. 2, Chemical and Biological Microsystems Society, pp. 820-822, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, Germany, 10/27/13.
Onoshima D, Kaji N, Tokeshi M, Baba Y. Microfluidic single-molecule nuclease digestion reveals rate-enhancing off-and-on molecular encountering function for site-specific DNA break. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society. 2013. p. 820-822. (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
Onoshima, D. ; Kaji, Noritada ; Tokeshi, M. ; Baba, Y. / Microfluidic single-molecule nuclease digestion reveals rate-enhancing off-and-on molecular encountering function for site-specific DNA break. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Chemical and Biological Microsystems Society, 2013. pp. 820-822 (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013).
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