TY - GEN
T1 - Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation
AU - Takeshita, Daiki
AU - Onoshima, Daisuke
AU - Hiroshi, Yukawa
AU - Yasui, Takao
AU - Kaji, Noritada
AU - Baba, Yoshinobu
PY - 2014/1/1
Y1 - 2014/1/1
N2 - We combined a microfluidic stretch of a single DNA molecule and a visualization technique with fluorescent gold nanoparticle labeling, which can be observed with fluorescence microscope and scanning electron microscope (SEM) for imaging of methylated site on a single DNA molecule. It will realize bimodal microscope imaging of methylated site and enhance the imaging resolution down to nanometer level toward epigenetic analysis on the basis of high-resolution mapping of methylation sites.
AB - We combined a microfluidic stretch of a single DNA molecule and a visualization technique with fluorescent gold nanoparticle labeling, which can be observed with fluorescence microscope and scanning electron microscope (SEM) for imaging of methylated site on a single DNA molecule. It will realize bimodal microscope imaging of methylated site and enhance the imaging resolution down to nanometer level toward epigenetic analysis on the basis of high-resolution mapping of methylation sites.
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UR - http://www.scopus.com/inward/citedby.url?scp=84941730442&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84941730442
T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
SP - 2348
EP - 2350
BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PB - Chemical and Biological Microsystems Society
T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Y2 - 26 October 2014 through 30 October 2014
ER -