Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation

Daiki Takeshita, Daisuke Onoshima, Yukawa Hiroshi, Takao Yasui, Noritada Kaji, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We combined a microfluidic stretch of a single DNA molecule and a visualization technique with fluorescent gold nanoparticle labeling, which can be observed with fluorescence microscope and scanning electron microscope (SEM) for imaging of methylated site on a single DNA molecule. It will realize bimodal microscope imaging of methylated site and enhance the imaging resolution down to nanometer level toward epigenetic analysis on the basis of high-resolution mapping of methylation sites.

Original languageEnglish
Title of host publication18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
PublisherChemical and Biological Microsystems Society
Pages2348-2350
Number of pages3
ISBN (Electronic)9780979806476
Publication statusPublished - Jan 1 2014
Externally publishedYes
Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 - San Antonio, United States
Duration: Oct 26 2014Oct 30 2014

Publication series

Name18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Other

Other18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
CountryUnited States
CitySan Antonio
Period10/26/1410/30/14

Fingerprint

Microfluidics
Stretching
DNA
Gold
Nanoparticles
Imaging techniques
Electrons
Microscopes
Molecules
Methylation
Labeling
Electron microscopes
Visualization
Fluorescence
Scanning
DNA Methylation

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Takeshita, D., Onoshima, D., Hiroshi, Y., Yasui, T., Kaji, N., & Baba, Y. (2014). Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 2348-2350). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation. / Takeshita, Daiki; Onoshima, Daisuke; Hiroshi, Yukawa; Yasui, Takao; Kaji, Noritada; Baba, Yoshinobu.

18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 2348-2350 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Takeshita, D, Onoshima, D, Hiroshi, Y, Yasui, T, Kaji, N & Baba, Y 2014, Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 2348-2350, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, San Antonio, United States, 10/26/14.
Takeshita D, Onoshima D, Hiroshi Y, Yasui T, Kaji N, Baba Y. Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 2348-2350. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
Takeshita, Daiki ; Onoshima, Daisuke ; Hiroshi, Yukawa ; Yasui, Takao ; Kaji, Noritada ; Baba, Yoshinobu. / Microfluidic stretching of DNA with fluorescent gold nanoparticle for optical/electron microscopic imaging of a single DNA methylation. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 2348-2350 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).
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