Microfluidic visualization of encounter complex in enzymatic digestion of single DNA molecule by dual molecular tagging

D. Onoshima, Noritada Kaji, M. Tokeshi, Y. Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We combined molecular tagging with microfluidics to study kinematics in a single DNA molecular digestion. Moving tags of both DNA ends revealed initial breakup of a single DNA molecule into fragments induced by limited enzymatic digestion and subsequent rapid formation of transient structure in encounter complex. The method enables us to analyze rapid dissociation of weakly bound complexes derived from intramolecular transfer.

Original languageEnglish
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages2061-2063
Number of pages3
Publication statusPublished - Dec 1 2011
Externally publishedYes
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume3

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Fingerprint

Microfluidics
DNA
Visualization
Molecules
Kinematics

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Onoshima, D., Kaji, N., Tokeshi, M., & Baba, Y. (2011). Microfluidic visualization of encounter complex in enzymatic digestion of single DNA molecule by dual molecular tagging. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 2061-2063). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).

Microfluidic visualization of encounter complex in enzymatic digestion of single DNA molecule by dual molecular tagging. / Onoshima, D.; Kaji, Noritada; Tokeshi, M.; Baba, Y.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 2061-2063 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 3).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Onoshima, D, Kaji, N, Tokeshi, M & Baba, Y 2011, Microfluidic visualization of encounter complex in enzymatic digestion of single DNA molecule by dual molecular tagging. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 3, pp. 2061-2063, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.
Onoshima D, Kaji N, Tokeshi M, Baba Y. Microfluidic visualization of encounter complex in enzymatic digestion of single DNA molecule by dual molecular tagging. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 2061-2063. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
Onoshima, D. ; Kaji, Noritada ; Tokeshi, M. ; Baba, Y. / Microfluidic visualization of encounter complex in enzymatic digestion of single DNA molecule by dual molecular tagging. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 2061-2063 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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