Microheater-integrated zinc oxide nanowire microfluidic device for hybridization-based detection of target single-stranded DNA

Hiromi Takahashi, Takao Yasui, Hiromu Kashida, Koki Makino, Keiko Shinjo, Quanli Liu, Taisuke Shimada, Sakon Rahong, Noritada Kaji, Hiroyuki Asanuma, Yoshinobu Baba

Research output: Contribution to journalArticlepeer-review

Abstract

Detection of cell-free DNA (cfDNA) has an impact on DNA analysis in liquid biopsies. However, current strategies to detect cfDNA have limitations that should be overcome, such as having low sensitivity and requiring much time and a specialized instrument. Thus, non-invasive and rapid detection tools are needed for disease prevention and early-stage treatment. Here we developed a device having a microheater integrated with zinc oxide nanowires (microheater-ZnO-NWs) to detect target single-stranded DNAs (ssDNAs) based on DNA probe hybridization. We confirmed experimentally that our device realized in-situ annealed DNA probes by which we subsequently detected target ssDNAs. We envision that this device can be utilized for fundamental studies related to nanobiodevice-based DNA detection.

Original languageEnglish
Article number255301
JournalNanotechnology
Volume32
Issue number25
DOIs
Publication statusPublished - Jun 18 2021

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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