Micropatterned saccharide display on the silicon surface

The immobilization and the micro-patterning of biomolecules on the solid surfaces are paid much attention for the application of biomaterials, and biosensors. Micro-patterned carbohydrates on solid surfaces are useful to analyze carbohydrate-protein interactions and to fabricate the scaffolds of cell cultivation. We have proposed a new method for micro-patterned carbohydrate display. The micropattern composed of hydrophobic and electrostatically positive regions were fabricated by methyl and amino terminated self-assembled monolayers (SAM). The patterned substrates were incubated in two types of aqueous solutions of PVLA and Heparin, in this order. The PVLA and Heparin site-selectively adsorbed on methyl and amino terminated SAMs, respectively. It indicated that the two dimensional matrix of PVLA and Heparin can be fabricated on the micro-patterned substrate. The micropatterning of two different components on one substrate enables two different biomacromolecules to be self-assembled into micro pattern.