Abstract
Sufficient vascularization is critical to sustaining viable tissue-engineered (TE) constructs after implantation. Despite significant progress, current approaches lack suturability, porosity, and biodegradability, which hinders rapid perfusion and remodeling in vivo. Consequently, TE vascular networks capable of direct anastomosis to host vasculature and immediate perfusion upon implantation still remain elusive. Here, a hybrid fabrication method is presented for micropatterning fibrous scaffolds that are suturable, porous, and biodegradable. Fused deposition modeling offers an inexpensive and automated approach to creating sacrificial templates with vascular-like branching. By electrospinning around these poly(vinyl alcohol) templates and dissolving them in water, microvascular patterns were transferred to fibrous scaffolds. Results indicated that these scaffolds have sufficient suture retention strength to permit direct anastomosis in future studies. Vascularization of these scaffolds is demonstrated by in vitro endothelialization and perfusion.
| Original language | English |
|---|---|
| Pages (from-to) | 1514-1520 |
| Number of pages | 7 |
| Journal | Macromolecular Bioscience |
| Volume | 14 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - Nov 1 2014 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Biomaterials
- Polymers and Plastics
- Materials Chemistry
Cite this
Micropatterning electrospun scaffolds to create intrinsic vascular networks. / Jeffries, Eric M.; Nakamura, Shintaro; Lee, Kee Won; Clampffer, Jimmy; Ijima, Hiroyuki; Wang, Yadong.
In: Macromolecular Bioscience, Vol. 14, No. 11, 01.11.2014, p. 1514-1520.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Micropatterning electrospun scaffolds to create intrinsic vascular networks
AU - Jeffries, Eric M.
AU - Nakamura, Shintaro
AU - Lee, Kee Won
AU - Clampffer, Jimmy
AU - Ijima, Hiroyuki
AU - Wang, Yadong
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Sufficient vascularization is critical to sustaining viable tissue-engineered (TE) constructs after implantation. Despite significant progress, current approaches lack suturability, porosity, and biodegradability, which hinders rapid perfusion and remodeling in vivo. Consequently, TE vascular networks capable of direct anastomosis to host vasculature and immediate perfusion upon implantation still remain elusive. Here, a hybrid fabrication method is presented for micropatterning fibrous scaffolds that are suturable, porous, and biodegradable. Fused deposition modeling offers an inexpensive and automated approach to creating sacrificial templates with vascular-like branching. By electrospinning around these poly(vinyl alcohol) templates and dissolving them in water, microvascular patterns were transferred to fibrous scaffolds. Results indicated that these scaffolds have sufficient suture retention strength to permit direct anastomosis in future studies. Vascularization of these scaffolds is demonstrated by in vitro endothelialization and perfusion.
AB - Sufficient vascularization is critical to sustaining viable tissue-engineered (TE) constructs after implantation. Despite significant progress, current approaches lack suturability, porosity, and biodegradability, which hinders rapid perfusion and remodeling in vivo. Consequently, TE vascular networks capable of direct anastomosis to host vasculature and immediate perfusion upon implantation still remain elusive. Here, a hybrid fabrication method is presented for micropatterning fibrous scaffolds that are suturable, porous, and biodegradable. Fused deposition modeling offers an inexpensive and automated approach to creating sacrificial templates with vascular-like branching. By electrospinning around these poly(vinyl alcohol) templates and dissolving them in water, microvascular patterns were transferred to fibrous scaffolds. Results indicated that these scaffolds have sufficient suture retention strength to permit direct anastomosis in future studies. Vascularization of these scaffolds is demonstrated by in vitro endothelialization and perfusion.
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UR - http://www.scopus.com/inward/citedby.url?scp=84924952615&partnerID=8YFLogxK
U2 - 10.1002/mabi.201400306
DO - 10.1002/mabi.201400306
M3 - Article
C2 - 25142314
AN - SCOPUS:84924952615
VL - 14
SP - 1514
EP - 1520
JO - Macromolecular Bioscience
JF - Macromolecular Bioscience
SN - 1616-5187
IS - 11
ER -