Micropropagation of ornamental Alocasia

Nguyen Thi Phuong Thao, Ikuo Miyajima, Kenji Ureshino, Yukio Ozaki, Hiroshi Okubo

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

In vitro multiplication of Alocasia micholitziana 'Green Velvet', Alocasia X amazonica and Alocasia cuculata was achieved from axillary buds excised from the corms of greenhouse-grown plants. Shoots were developed directly within 6-8 weeks on the MS medium supplemented with 10 mgl -1 2-iP for A. micholitziana 'Green Velvet' or 10 mgl -1 BA for A. X amazonica and A. cuculata. Considering the quality of the shoots, high frequency of shoot regeneration was achieved on similar medium in subsequent subcultures for A. micholitziana 'Green Velvet' (5.0 shoots/culture). Five mgl -1 BA gave the best shoot regeneration in A. X amazonica and A. cuculata (4.6 and 5.0 shoots/culture, respectively). Shoots of the regenerated plants rooted abundantly on the hormone free MS within 4 weeks. Acclimatization was successful with approximately 100% of the in vitro plantlets which survived in a greenhouse. The regenerated plants seemed morphologically to be similar to the respective mother plants. The established procedure provided a basic technique to carry out the furture in vitro experiments in Alocasia.

Original languageEnglish
Pages (from-to)277-282
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume47
Issue number2
Publication statusPublished - Feb 1 2003

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

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