MicroRNAs induce epigenetic reprogramming and suppress malignant phenotypes of human colon cancer cells

Hisataka Ogawa, Xin Wu, Koichi Kawamoto, Naohiro Nishida, Masamitsu Konno, Jun Koseki, Hidetoshi Matsui, Kozou Noguchi, Noriko Gotoh, Tsuyoshi Yamamoto, Kanjiro Miyata, Nobuhiro Nishiyama, Hiroaki Nagano, Hirofumi Yamamoto, Satoshi Obika, Kazunori Kataoka, Yuichiro Doki, Masaki Mori, Hideshi Ishii

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Although cancer is a genetic disease, epigenetic alterations are involved in its initiation and progression. Previous studies have shown that reprogramming of colon cancer cells using Oct3/4, Sox2, Klf4, and cMyc reduces cancer malignancy. Therefore, cancer reprogramming may be a useful treatment for chemo- or radiotherapy-resistant cancer cells. It was also reported that the introduction of endogenous small-sized, non-coding ribonucleotides such as microRNA (miR) 302s and miR-369-3p or -5p resulted in the induction of cellular reprogramming. miRs are smaller than the genes of transcription factors, making them possibly suitable for use in clinical strategies. Therefore, we reprogrammed colon cancer cells using miR-302s and miR-369-3p or -5p. This resulted in inhibition of cell proliferation and invasion and the stimulation of the mesenchymal-to-epithelial transition phenotype in colon cancer cells. Importantly, the introduction of the ribonucleotides resulted in epigenetic reprogramming of DNA demethylation and histone modification events. Furthermore, in vivo administration of the ribonucleotides in mice elicited the induction of cancer cell apoptosis, which involves the mitochondrial Bcl2 protein family. The present study shows that the introduction of miR-302s and miR-369s could induce cellular reprogramming and modulate malignant phenotypes of human colorectal cancer, suggesting that the appropriate delivery of functional small-sized ribonucleotides may open a new avenue for therapy against human malignant tumors.

Original languageEnglish
Article numbere0127119
JournalPloS one
Volume10
Issue number5
DOIs
Publication statusPublished - May 13 2015

Fingerprint

colorectal neoplasms
MicroRNAs
microRNA
Epigenomics
epigenetics
ribonucleotides
Colonic Neoplasms
Ribonucleotides
Cells
Phenotype
phenotype
Neoplasms
neoplasms
Histone Code
cell invasion
Chemotherapy
Radiotherapy
Cell proliferation
genetic disorders
radiotherapy

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Ogawa, H., Wu, X., Kawamoto, K., Nishida, N., Konno, M., Koseki, J., ... Ishii, H. (2015). MicroRNAs induce epigenetic reprogramming and suppress malignant phenotypes of human colon cancer cells. PloS one, 10(5), [e0127119]. https://doi.org/10.1371/journal.pone.0127119

MicroRNAs induce epigenetic reprogramming and suppress malignant phenotypes of human colon cancer cells. / Ogawa, Hisataka; Wu, Xin; Kawamoto, Koichi; Nishida, Naohiro; Konno, Masamitsu; Koseki, Jun; Matsui, Hidetoshi; Noguchi, Kozou; Gotoh, Noriko; Yamamoto, Tsuyoshi; Miyata, Kanjiro; Nishiyama, Nobuhiro; Nagano, Hiroaki; Yamamoto, Hirofumi; Obika, Satoshi; Kataoka, Kazunori; Doki, Yuichiro; Mori, Masaki; Ishii, Hideshi.

In: PloS one, Vol. 10, No. 5, e0127119, 13.05.2015.

Research output: Contribution to journalArticle

Ogawa, H, Wu, X, Kawamoto, K, Nishida, N, Konno, M, Koseki, J, Matsui, H, Noguchi, K, Gotoh, N, Yamamoto, T, Miyata, K, Nishiyama, N, Nagano, H, Yamamoto, H, Obika, S, Kataoka, K, Doki, Y, Mori, M & Ishii, H 2015, 'MicroRNAs induce epigenetic reprogramming and suppress malignant phenotypes of human colon cancer cells', PloS one, vol. 10, no. 5, e0127119. https://doi.org/10.1371/journal.pone.0127119
Ogawa, Hisataka ; Wu, Xin ; Kawamoto, Koichi ; Nishida, Naohiro ; Konno, Masamitsu ; Koseki, Jun ; Matsui, Hidetoshi ; Noguchi, Kozou ; Gotoh, Noriko ; Yamamoto, Tsuyoshi ; Miyata, Kanjiro ; Nishiyama, Nobuhiro ; Nagano, Hiroaki ; Yamamoto, Hirofumi ; Obika, Satoshi ; Kataoka, Kazunori ; Doki, Yuichiro ; Mori, Masaki ; Ishii, Hideshi. / MicroRNAs induce epigenetic reprogramming and suppress malignant phenotypes of human colon cancer cells. In: PloS one. 2015 ; Vol. 10, No. 5.
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