Migration of Thy-1+ dendritic epidermal cells (Thy-1+DEC): Ly48 and TNF-α are responsible for the migration of Thy-1+DEC to the epidermis

Kunihiko Tamaki, Atsushi Saitoh, Anthony A. Gaspari, Nami Yasaka, Masutaka Furue

Research output: Contribution to journalArticle

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Abstract

Thy-1+ dendritic epidermal cells (Thy-1+DEC) are mainly T cells that express T-cell receptor γ and δ chains with limited diversity of γδ, mainly γ3δ1; such γ3δ1 TCR-bearing Thy-1+DEC originate from day 16 fetal thymic cells. To understand the migratory capability of Thy-1+DEC, we developed an in vitro model, using skin organ culture. First, emigration of Thy1+DEC from the epidermis was examined. Ear skin from C3H/He mice was separated into two parts and incubated for 3 d with dermal side down. Thy-1+DEC emigrated from the epidermis into the dermis and then migrated out of the skin into the culture medium. Next, immigration of Thy-1+DEC into the epidermis was examined. Thy-1+DEC were depleted in vivo by daily application of clobetazole propionate solution topically onto the ears of C3H/He mice. Seven days later, ear skin was harvested, separated, and cultured with the dermal side up with syngeneic epidermal cell suspensions with a migration chamber for 3 d. It was found that 1) Thy-1+DEC immigrated into the Thy-1+DEC depleted epidermis as well as into untreated epidermis, and 2) the migratory capability of Thy-1+DEC was directly proved by a biolabeling technique with PKH-26. Blocking studies with various antibodies revealed that leukosialin (S11 monoclonal antibodies) and TNFα were important for Thy-1+DEC migration. Thus, Thy-1+DEC retain the potential for migration in vitro, and leukosialin and TNFα are partially responsible for the migration of Thy-1+DEC into the epidermis.

Original languageEnglish
Pages (from-to)290-294
Number of pages5
JournalJournal of Investigative Dermatology
Volume103
Issue number3
DOIs
Publication statusPublished - Jan 1 1994
Externally publishedYes

Fingerprint

Langerhans Cells
Epidermis
CD43 Antigens
Skin
Bearings (structural)
T-cells
Propionates
T-Cell Antigen Receptor
Ear
Culture Media
Inbred C3H Mouse
Suspensions
Emigration and Immigration
Monoclonal Antibodies
Antibodies
Organ Culture Techniques
Dermis
Cell Movement
T-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

Cite this

Migration of Thy-1+ dendritic epidermal cells (Thy-1+DEC) : Ly48 and TNF-α are responsible for the migration of Thy-1+DEC to the epidermis. / Tamaki, Kunihiko; Saitoh, Atsushi; Gaspari, Anthony A.; Yasaka, Nami; Furue, Masutaka.

In: Journal of Investigative Dermatology, Vol. 103, No. 3, 01.01.1994, p. 290-294.

Research output: Contribution to journalArticle

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abstract = "Thy-1+ dendritic epidermal cells (Thy-1+DEC) are mainly T cells that express T-cell receptor γ and δ chains with limited diversity of γδ, mainly γ3δ1; such γ3δ1 TCR-bearing Thy-1+DEC originate from day 16 fetal thymic cells. To understand the migratory capability of Thy-1+DEC, we developed an in vitro model, using skin organ culture. First, emigration of Thy1+DEC from the epidermis was examined. Ear skin from C3H/He mice was separated into two parts and incubated for 3 d with dermal side down. Thy-1+DEC emigrated from the epidermis into the dermis and then migrated out of the skin into the culture medium. Next, immigration of Thy-1+DEC into the epidermis was examined. Thy-1+DEC were depleted in vivo by daily application of clobetazole propionate solution topically onto the ears of C3H/He mice. Seven days later, ear skin was harvested, separated, and cultured with the dermal side up with syngeneic epidermal cell suspensions with a migration chamber for 3 d. It was found that 1) Thy-1+DEC immigrated into the Thy-1+DEC depleted epidermis as well as into untreated epidermis, and 2) the migratory capability of Thy-1+DEC was directly proved by a biolabeling technique with PKH-26. Blocking studies with various antibodies revealed that leukosialin (S11 monoclonal antibodies) and TNFα were important for Thy-1+DEC migration. Thus, Thy-1+DEC retain the potential for migration in vitro, and leukosialin and TNFα are partially responsible for the migration of Thy-1+DEC into the epidermis.",
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