Migratory activity of CD105+ pancreatic cancer cells is strongly enhanced by pancreatic stellate cells

Kenji Fujiwara, Kenoki Ouchida, Ohtsuka Takao, Kazuhiro Mizumoto, Koji Shindo, Naoki Ikenaga, Lin Cui, Shunichi Takahata, Shinichi Aishima, Masao Tanaka

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

OBJECTIVES: CD105 expression correlates with prognosis for several cancers. However, its significance in pancreatic cancer is unclear. METHODS: We analyzed CD105 expression in resected pancreatic cancer tissue and pancreatic cancer cell lines, compared the properties of CD105 and CD105 cells using quantitative RT-PCR and migration assays, and evaluated the relationship between CD105 cells and pancreatic stellate cells (PSCs). RESULTS: Immunohistochemistry showed that the frequency of CD105 expression was higher in pancreatic cancer than that in normal tissue (8% vs 0%, respectively). In flow cytometry, CD105 was expressed in pancreatic cancer cells, whereas weak CD105 expression was detected in normal pancreatic ductal epithelial cells. Quantitative RT-PCR showed that E-cadherin mRNA expression was suppressed and vimentin mRNA was overexpressed in CD105 cells (P < 0.05). Migration of CD105 cancer cells was strongly enhanced (more than that of CD105 cells) in coculture with PSCs (P < 0.05). CD105 expression did not correlate to clinicopathologic characteristics or the Kaplan-Meier survival analysis. CONCLUSIONS: Suppression of an epithelial marker and overexpression of a mesenchymal marker suggest that epithelial-mesenchymal transition is induced in CD105 pancreatic cancer cells. CD105 pancreatic cancer cell migration is strongly enhanced by PSCs, suggesting that these cells play a role in the pancreatic cancer microenvironment.

Original languageEnglish
Pages (from-to)1283-1290
Number of pages8
JournalPancreas
Volume42
Issue number8
DOIs
Publication statusPublished - Nov 1 2013

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Pancreatic Stellate Cells
Pancreatic Neoplasms
Polymerase Chain Reaction
Messenger RNA
Epithelial-Mesenchymal Transition
Tumor Microenvironment
Kaplan-Meier Estimate
Vimentin
Cadherins
Survival Analysis
Coculture Techniques
Cell Movement
Neoplasms
Flow Cytometry
Epithelial Cells
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Hepatology
  • Endocrinology

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Migratory activity of CD105+ pancreatic cancer cells is strongly enhanced by pancreatic stellate cells. / Fujiwara, Kenji; Ouchida, Kenoki; Takao, Ohtsuka; Mizumoto, Kazuhiro; Shindo, Koji; Ikenaga, Naoki; Cui, Lin; Takahata, Shunichi; Aishima, Shinichi; Tanaka, Masao.

In: Pancreas, Vol. 42, No. 8, 01.11.2013, p. 1283-1290.

Research output: Contribution to journalArticle

Fujiwara, Kenji ; Ouchida, Kenoki ; Takao, Ohtsuka ; Mizumoto, Kazuhiro ; Shindo, Koji ; Ikenaga, Naoki ; Cui, Lin ; Takahata, Shunichi ; Aishima, Shinichi ; Tanaka, Masao. / Migratory activity of CD105+ pancreatic cancer cells is strongly enhanced by pancreatic stellate cells. In: Pancreas. 2013 ; Vol. 42, No. 8. pp. 1283-1290.
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AU - Shindo, Koji

AU - Ikenaga, Naoki

AU - Cui, Lin

AU - Takahata, Shunichi

AU - Aishima, Shinichi

AU - Tanaka, Masao

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