Milk fermented by Lactobacillus gasseri SBT2055 influences adipocyte size via inhibition of dietary fat absorption in Zucker rats

Essam M. Hamad, Masao Sato, Kazunori Uzu, Takeshi Yoshida, Seiichiro Higashi, Hiroshi Kawakami, Yukio Kadooka, Hiroaki Matsuyama, Ibrahim A.Abd El-Gawad, Katsumi Imaizumi

Research output: Contribution to journalArticle

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Abstract

We have demonstrated previously that a diet containing skimmed milk (SM) fermented by Lactobacillus gasseri SBT2055 (LGSP) reduces adipocyte size in Sprague-Dawley rats. Two experiments were conducted to extend these observations in order to elucidate the mechanism involved. In experiment 1, lean and obese Zucker rats were fed a diet containing SM or LGSP for 4 weeks. The LGSP diet, compared with the SM diet, resulted in lowering of the mesenteric adipose tissue weight (23 %; P<0.05), adipocyte sizes (28 %; P<0.001) and serum leptin concentration (36 %; P<0.05) in lean rats. Obese Zucker rats did not display such dietary effects. Only the number of smaller adipocytes was increased (P<0.05) by the LGSP diet in the subcutaneous adipose tissue of obese rats. The LGSP diet significantly reduced the serum and hepatic cholesterol in rats. In addition, the LGSP diet led to an increased excretion of faecal fatty acids and total neutral faecal sterols in both rat strains. In experiment 2, Sprague-Dawley rats with permanent cannulation of the thoracic duct were fed either the SM or LGSP diets and their lymph was collected. The LGSP diet lowered the maximum transport rate of TAG and phospholipids. These results indicate that fermented milk regulates adipose tissue growth through inhibition at the stage of dietary fat absorption in lean Zucker rats.

Original languageEnglish
Pages (from-to)716-724
Number of pages9
JournalBritish Journal of Nutrition
Volume101
Issue number5
DOIs
Publication statusPublished - Jul 3 2009

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Zucker Rats
Dietary Fats
Adipocytes
Milk
Diet
Sprague Dawley Rats
Adipose Tissue
Lactobacillus gasseri
Thoracic Duct
Subcutaneous Fat
Lymph
Sterols
Leptin
Serum
Catheterization
Phospholipids
Fatty Acids
Cholesterol
Weights and Measures

All Science Journal Classification (ASJC) codes

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Milk fermented by Lactobacillus gasseri SBT2055 influences adipocyte size via inhibition of dietary fat absorption in Zucker rats. / Hamad, Essam M.; Sato, Masao; Uzu, Kazunori; Yoshida, Takeshi; Higashi, Seiichiro; Kawakami, Hiroshi; Kadooka, Yukio; Matsuyama, Hiroaki; El-Gawad, Ibrahim A.Abd; Imaizumi, Katsumi.

In: British Journal of Nutrition, Vol. 101, No. 5, 03.07.2009, p. 716-724.

Research output: Contribution to journalArticle

Hamad, EM, Sato, M, Uzu, K, Yoshida, T, Higashi, S, Kawakami, H, Kadooka, Y, Matsuyama, H, El-Gawad, IAA & Imaizumi, K 2009, 'Milk fermented by Lactobacillus gasseri SBT2055 influences adipocyte size via inhibition of dietary fat absorption in Zucker rats', British Journal of Nutrition, vol. 101, no. 5, pp. 716-724. https://doi.org/10.1017/S0007114508043808
Hamad, Essam M. ; Sato, Masao ; Uzu, Kazunori ; Yoshida, Takeshi ; Higashi, Seiichiro ; Kawakami, Hiroshi ; Kadooka, Yukio ; Matsuyama, Hiroaki ; El-Gawad, Ibrahim A.Abd ; Imaizumi, Katsumi. / Milk fermented by Lactobacillus gasseri SBT2055 influences adipocyte size via inhibition of dietary fat absorption in Zucker rats. In: British Journal of Nutrition. 2009 ; Vol. 101, No. 5. pp. 716-724.
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