Minimal requirements for the nuclear localization of p27(Kip1), a cyclin-dependent kinase inhibitor

Ying Zeng, Katsuya Hirano, Mayumi Hirano, Junji Nishimura, Hideo Kanaide

Research output: Contribution to journalArticle

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Abstract

p27(Kip1) is a cyclin-dependent kinase inhibitor, and its nuclear localization is a prerequisite for it to function as a cell cycle regulator. In the present study, the minimal requirement for the nuclear localization signal (NLS) of p27(Kip1) was determined by analyzing the localization of various mutants of p27(Kip1) tagged with green fluorescent protein (GFP) in HeLa cells and porcine aortic endothelial cells. Wild-type p27(Kip1) exclusively localized into nucleus, while GFP alone localized in both cytosol and nucleus. A comparison of various truncation mutants revealed residues 153-166 to be the minimal region necessary for nuclear localization. However, a fusion of this region to GFP showed cytoplasmic retention in addition to nuclear localization, thus suggesting that some extension flanking this region is required to achieve a full function of NLS. The site-directed mutation of the full-length p27(Kip1) therefore showed that four basic residues (K153, R154, K165, R166), especially R166, play a critical role in the nuclear localization of p27(Kip1). (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume274
Issue number1
DOIs
Publication statusPublished - Jul 21 2000

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Cyclin-Dependent Kinases
Green Fluorescent Proteins
Nuclear Localization Signals
Endothelial cells
HeLa Cells
Cytosol
Cell Cycle
Swine
Fusion reactions
Endothelial Cells
Cells
Mutation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Minimal requirements for the nuclear localization of p27(Kip1), a cyclin-dependent kinase inhibitor. / Zeng, Ying; Hirano, Katsuya; Hirano, Mayumi; Nishimura, Junji; Kanaide, Hideo.

In: Biochemical and Biophysical Research Communications, Vol. 274, No. 1, 21.07.2000, p. 37-42.

Research output: Contribution to journalArticle

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AB - p27(Kip1) is a cyclin-dependent kinase inhibitor, and its nuclear localization is a prerequisite for it to function as a cell cycle regulator. In the present study, the minimal requirement for the nuclear localization signal (NLS) of p27(Kip1) was determined by analyzing the localization of various mutants of p27(Kip1) tagged with green fluorescent protein (GFP) in HeLa cells and porcine aortic endothelial cells. Wild-type p27(Kip1) exclusively localized into nucleus, while GFP alone localized in both cytosol and nucleus. A comparison of various truncation mutants revealed residues 153-166 to be the minimal region necessary for nuclear localization. However, a fusion of this region to GFP showed cytoplasmic retention in addition to nuclear localization, thus suggesting that some extension flanking this region is required to achieve a full function of NLS. The site-directed mutation of the full-length p27(Kip1) therefore showed that four basic residues (K153, R154, K165, R166), especially R166, play a critical role in the nuclear localization of p27(Kip1). (C) 2000 Academic Press.

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