Minimum essential region of CCG1/TAFII250 required for complementing the temperature-sensitive cell cycle mutants, tsBN462 and ts13 cells, of hamster BHK21 cells

Eishi Noguchi, Takeshi Sekiguchi, Yukiko Nohiro, Toshiro Hayashida, Eiji Hirose, Naoyuki Hayashi, Takeharu Nishimoto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

CCG1/TAFII250, the largest subunit of theTFIID complex, is mutated in ts cell cycle mutants of BHK21 cells, ts13 and tsBN462, which have a promoter-selective transcriptional defect. A series of deletion mutants of CCG1 cDNA were prepared and transfected into these mutants, in order to identify functional domains of CCG1 required for the complementation of ts 13/BN462 mutation. We determined the minimum size of CCG1: CCG1ME, essential for complementing the ts mutation, which possessed one proline cluster, an HMG1-like domain, and a nuclear localization signal, but which lacked the bromo domains and the acidic phosphorylation sites for casein kinase II common to transcriptional activators. It encodes a protein of 140 kDa. These characteristics of CCG1ME correspond to yeast TAFII145, the yeast homolog of human TAFII250. CCG1ME bound to TBP, creating its own TFIID complex different from that of the endogenous mutated CCG1 in ts+ transformants of tsBN462 cells.

Original languageEnglish
Pages (from-to)505-513
Number of pages9
JournalSomatic Cell and Molecular Genetics
Volume20
Issue number6
DOIs
Publication statusPublished - Nov 1 1994

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Cricetinae
Cell Cycle
Yeasts
Transcription Factor TFIID
HMGB1 Protein
Casein Kinase II
Nuclear Localization Signals
Mutation
Temperature
Proline
Complementary DNA
Phosphorylation
Proteins
tributyl phosphate

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this

Minimum essential region of CCG1/TAFII250 required for complementing the temperature-sensitive cell cycle mutants, tsBN462 and ts13 cells, of hamster BHK21 cells. / Noguchi, Eishi; Sekiguchi, Takeshi; Nohiro, Yukiko; Hayashida, Toshiro; Hirose, Eiji; Hayashi, Naoyuki; Nishimoto, Takeharu.

In: Somatic Cell and Molecular Genetics, Vol. 20, No. 6, 01.11.1994, p. 505-513.

Research output: Contribution to journalArticle

Noguchi, Eishi ; Sekiguchi, Takeshi ; Nohiro, Yukiko ; Hayashida, Toshiro ; Hirose, Eiji ; Hayashi, Naoyuki ; Nishimoto, Takeharu. / Minimum essential region of CCG1/TAFII250 required for complementing the temperature-sensitive cell cycle mutants, tsBN462 and ts13 cells, of hamster BHK21 cells. In: Somatic Cell and Molecular Genetics. 1994 ; Vol. 20, No. 6. pp. 505-513.
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abstract = "CCG1/TAFII250, the largest subunit of theTFIID complex, is mutated in ts cell cycle mutants of BHK21 cells, ts13 and tsBN462, which have a promoter-selective transcriptional defect. A series of deletion mutants of CCG1 cDNA were prepared and transfected into these mutants, in order to identify functional domains of CCG1 required for the complementation of ts 13/BN462 mutation. We determined the minimum size of CCG1: CCG1ME, essential for complementing the ts mutation, which possessed one proline cluster, an HMG1-like domain, and a nuclear localization signal, but which lacked the bromo domains and the acidic phosphorylation sites for casein kinase II common to transcriptional activators. It encodes a protein of 140 kDa. These characteristics of CCG1ME correspond to yeast TAFII145, the yeast homolog of human TAFII250. CCG1ME bound to TBP, creating its own TFIID complex different from that of the endogenous mutated CCG1 in ts+ transformants of tsBN462 cells.",
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