Abstract
CCG1/TAFII250, the largest subunit of theTFIID complex, is mutated in ts cell cycle mutants of BHK21 cells, ts13 and tsBN462, which have a promoter-selective transcriptional defect. A series of deletion mutants of CCG1 cDNA were prepared and transfected into these mutants, in order to identify functional domains of CCG1 required for the complementation of ts 13/BN462 mutation. We determined the minimum size of CCG1: CCG1ME, essential for complementing the ts mutation, which possessed one proline cluster, an HMG1-like domain, and a nuclear localization signal, but which lacked the bromo domains and the acidic phosphorylation sites for casein kinase II common to transcriptional activators. It encodes a protein of 140 kDa. These characteristics of CCG1ME correspond to yeast TAFII145, the yeast homolog of human TAFII250. CCG1ME bound to TBP, creating its own TFIID complex different from that of the endogenous mutated CCG1 in ts+ transformants of tsBN462 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 505-513 |
| Number of pages | 9 |
| Journal | Somatic Cell and Molecular Genetics |
| Volume | 20 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Nov 1 1994 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Genetics
- Cell Biology
Cite this
Minimum essential region of CCG1/TAFII250 required for complementing the temperature-sensitive cell cycle mutants, tsBN462 and ts13 cells, of hamster BHK21 cells. / Noguchi, Eishi; Sekiguchi, Takeshi; Nohiro, Yukiko; Hayashida, Toshiro; Hirose, Eiji; Hayashi, Naoyuki; Nishimoto, Takeharu.
In: Somatic Cell and Molecular Genetics, Vol. 20, No. 6, 01.11.1994, p. 505-513.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Minimum essential region of CCG1/TAFII250 required for complementing the temperature-sensitive cell cycle mutants, tsBN462 and ts13 cells, of hamster BHK21 cells
AU - Noguchi, Eishi
AU - Sekiguchi, Takeshi
AU - Nohiro, Yukiko
AU - Hayashida, Toshiro
AU - Hirose, Eiji
AU - Hayashi, Naoyuki
AU - Nishimoto, Takeharu
PY - 1994/11/1
Y1 - 1994/11/1
N2 - CCG1/TAFII250, the largest subunit of theTFIID complex, is mutated in ts cell cycle mutants of BHK21 cells, ts13 and tsBN462, which have a promoter-selective transcriptional defect. A series of deletion mutants of CCG1 cDNA were prepared and transfected into these mutants, in order to identify functional domains of CCG1 required for the complementation of ts 13/BN462 mutation. We determined the minimum size of CCG1: CCG1ME, essential for complementing the ts mutation, which possessed one proline cluster, an HMG1-like domain, and a nuclear localization signal, but which lacked the bromo domains and the acidic phosphorylation sites for casein kinase II common to transcriptional activators. It encodes a protein of 140 kDa. These characteristics of CCG1ME correspond to yeast TAFII145, the yeast homolog of human TAFII250. CCG1ME bound to TBP, creating its own TFIID complex different from that of the endogenous mutated CCG1 in ts+ transformants of tsBN462 cells.
AB - CCG1/TAFII250, the largest subunit of theTFIID complex, is mutated in ts cell cycle mutants of BHK21 cells, ts13 and tsBN462, which have a promoter-selective transcriptional defect. A series of deletion mutants of CCG1 cDNA were prepared and transfected into these mutants, in order to identify functional domains of CCG1 required for the complementation of ts 13/BN462 mutation. We determined the minimum size of CCG1: CCG1ME, essential for complementing the ts mutation, which possessed one proline cluster, an HMG1-like domain, and a nuclear localization signal, but which lacked the bromo domains and the acidic phosphorylation sites for casein kinase II common to transcriptional activators. It encodes a protein of 140 kDa. These characteristics of CCG1ME correspond to yeast TAFII145, the yeast homolog of human TAFII250. CCG1ME bound to TBP, creating its own TFIID complex different from that of the endogenous mutated CCG1 in ts+ transformants of tsBN462 cells.
UR - http://www.scopus.com/inward/record.url?scp=0028627735&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028627735&partnerID=8YFLogxK
U2 - 10.1007/BF02255841
DO - 10.1007/BF02255841
M3 - Article
C2 - 7892648
AN - SCOPUS:0028627735
VL - 20
SP - 505
EP - 513
JO - Somatic Cell and Molecular Genetics
JF - Somatic Cell and Molecular Genetics
SN - 0740-7750
IS - 6
ER -