miR-203 inhibits Frizzled-2 expression via CD82/KAI1 expression in human lung carcinoma cells

Mariko Mine, Kojiro Yamaguchi, Tsuyoshi Sugiura, Satomi Chigita, Naoya Yoshihama, Rumi Yoshihama, Naomi Hiyake, Yosuke Kobayashi, Yoshihide Mori

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

CD82/KAI1, a member of the tetraspanin superfamily, is a suppressor of metastasis and CD82 inhibits canonical Wnt signaling via downregulation of several Frizzled (FZD) isoforms, resulting in accumulation of β-catenin at the cell membrane. In this study, we investigated the mechanism through which CD82 inhibited FZD expression by examining the effects of microRNAs (miRNAs). The miRanda algorithm predicted 11 miRNAs from FZD sequences. Among these miRNAs, CD82 caused upregulation of miR-203 (by 2.095-fold) and downregulation of miR-338-3p (by 0.354-fold) as compared with control cells. Transfection with miR-203 and miR338-3p mimics or inhibitors revealed that miR-203 downregulated FZD2 mRNA (by 0.268-fold) and protein expression (by 0.701-fold). Moreover, transfection with the miR-203 mimic also inhibited cell migration. Therefore, these findings suggested that CD82 enhanced the expression of miR-203 and directly downregulate FZD2 expression, suppressing cancer metastasis by inhibition of the Wnt signaling pathway.

Original languageEnglish
Article numbere0131350
JournalPloS one
Volume10
Issue number7
DOIs
Publication statusPublished - Jul 1 2015

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MicroRNAs
microRNA
carcinoma
Down-Regulation
lungs
Cells
transfection
Carcinoma
metastasis
Lung
Transfection
Catenins
cells
Cell membranes
Neoplasm Metastasis
cell movement
Wnt Signaling Pathway
cell membranes
Protein Isoforms
protein synthesis

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Mine, M., Yamaguchi, K., Sugiura, T., Chigita, S., Yoshihama, N., Yoshihama, R., ... Mori, Y. (2015). miR-203 inhibits Frizzled-2 expression via CD82/KAI1 expression in human lung carcinoma cells. PloS one, 10(7), [e0131350]. https://doi.org/10.1371/journal.pone.0131350

miR-203 inhibits Frizzled-2 expression via CD82/KAI1 expression in human lung carcinoma cells. / Mine, Mariko; Yamaguchi, Kojiro; Sugiura, Tsuyoshi; Chigita, Satomi; Yoshihama, Naoya; Yoshihama, Rumi; Hiyake, Naomi; Kobayashi, Yosuke; Mori, Yoshihide.

In: PloS one, Vol. 10, No. 7, e0131350, 01.07.2015.

Research output: Contribution to journalArticle

Mine, M, Yamaguchi, K, Sugiura, T, Chigita, S, Yoshihama, N, Yoshihama, R, Hiyake, N, Kobayashi, Y & Mori, Y 2015, 'miR-203 inhibits Frizzled-2 expression via CD82/KAI1 expression in human lung carcinoma cells', PloS one, vol. 10, no. 7, e0131350. https://doi.org/10.1371/journal.pone.0131350
Mine M, Yamaguchi K, Sugiura T, Chigita S, Yoshihama N, Yoshihama R et al. miR-203 inhibits Frizzled-2 expression via CD82/KAI1 expression in human lung carcinoma cells. PloS one. 2015 Jul 1;10(7). e0131350. https://doi.org/10.1371/journal.pone.0131350
Mine, Mariko ; Yamaguchi, Kojiro ; Sugiura, Tsuyoshi ; Chigita, Satomi ; Yoshihama, Naoya ; Yoshihama, Rumi ; Hiyake, Naomi ; Kobayashi, Yosuke ; Mori, Yoshihide. / miR-203 inhibits Frizzled-2 expression via CD82/KAI1 expression in human lung carcinoma cells. In: PloS one. 2015 ; Vol. 10, No. 7.
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