miR-3148 is a novel onco-microRNA that potentiates tumor growth in vivo

Takaki Akamine, Yosuke Morodomi, Yui Harada, Koji Teraishi, Tetsuzo Tagawa, Tatsuro Okamoto, Yoshihiko Maehara, Yoshikazu Yonemitsu

Research output: Contribution to journalArticle

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Abstract

Background/Aim: Alterations of microRNA expression in three-dimensional spheroids were examined to identify novel microRNAs that might be associated with tumorigenesis. Materials and Methods: Using microRNA microarray analysis, we screened for microRNAs that were dramatically up-regulated inside three-dimensional spheroids in genetically-modified HCT116 human colon cancer cells expressing Copepoda Green Fluorescent Protein under hypoxia. Results: miR-3148 was identified as a possible candidate onco-microRNA. A growth advantage of HCT116 cells stably expressing miR-3148 (HCT116-miR3148) was observed compared to parental cells in vivo, but not in vitro. Additionally, no change in growth under hypoxic or starvation conditions was seen in these cells cultured two-dimensionally; however, HCT116-miR3148 cells maintained as three-dimensional spheroids were highly resistant to hypoxic conditions. HCT116-miR3148 cells were more sensitive to mitogen-activated protein kinase (MAPK) kinase inhibitors and extracellular signal-regulated kinase (ERK) inhibitors. Conclusion: MiR-3148 may be a novel onco-microRNA that protects cancer cells from serious stress conditions through the MAPK/ERK pathway, especially in vivo.

Original languageEnglish
Pages (from-to)5693-5701
Number of pages9
JournalAnticancer research
Volume38
Issue number10
DOIs
Publication statusPublished - Oct 1 2018

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MicroRNAs
HCT116 Cells
Growth
Neoplasms
Extracellular Signal-Regulated MAP Kinases
Copepoda
Mitogen-Activated Protein Kinase Kinases
Microarray Analysis
Starvation
Green Fluorescent Proteins
Mitogen-Activated Protein Kinases
Colonic Neoplasms
Cultured Cells
Carcinogenesis

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

miR-3148 is a novel onco-microRNA that potentiates tumor growth in vivo. / Akamine, Takaki; Morodomi, Yosuke; Harada, Yui; Teraishi, Koji; Tagawa, Tetsuzo; Okamoto, Tatsuro; Maehara, Yoshihiko; Yonemitsu, Yoshikazu.

In: Anticancer research, Vol. 38, No. 10, 01.10.2018, p. 5693-5701.

Research output: Contribution to journalArticle

Akamine, Takaki ; Morodomi, Yosuke ; Harada, Yui ; Teraishi, Koji ; Tagawa, Tetsuzo ; Okamoto, Tatsuro ; Maehara, Yoshihiko ; Yonemitsu, Yoshikazu. / miR-3148 is a novel onco-microRNA that potentiates tumor growth in vivo. In: Anticancer research. 2018 ; Vol. 38, No. 10. pp. 5693-5701.
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