TY - JOUR
T1 - miR-3148 is a novel onco-microRNA that potentiates tumor growth in vivo
AU - Akamine, Takaki
AU - Morodomi, Yosuke
AU - Harada, Yui
AU - Teraishi, Koji
AU - Tagawa, Tetsuzo
AU - Okamoto, Tatsuro
AU - Maehara, Yoshihiko
AU - Yonemitsu, Yoshikazu
N1 - Funding Information:
The Authors thank Naomi Ono, Risa Tanaka, Ryoko Nakamura, and Natsumi Maeda (R&D Laboratory for Innovative bioTherapeutics Science) for technical assistance. The Authors also acknowledge the gift of CH4987655 from Chugai Pharmaceutical Co. The Authors also thank Sarah Williams, PhD, from Edanz Group (www.edanzediting.com) for editing a draft of this manuscript. This work was supported by MEXT KAKENHI (Grant Number 15H05792) and the KAIBARA MORIKAZU MEDICAL SCIENCE PROMOTION FOUNDATION.
Publisher Copyright:
© 2018 International Institute of Anticancer Research. All rights reserved.
PY - 2018/10
Y1 - 2018/10
N2 - Background/Aim: Alterations of microRNA expression in three-dimensional spheroids were examined to identify novel microRNAs that might be associated with tumorigenesis. Materials and Methods: Using microRNA microarray analysis, we screened for microRNAs that were dramatically up-regulated inside three-dimensional spheroids in genetically-modified HCT116 human colon cancer cells expressing Copepoda Green Fluorescent Protein under hypoxia. Results: miR-3148 was identified as a possible candidate onco-microRNA. A growth advantage of HCT116 cells stably expressing miR-3148 (HCT116-miR3148) was observed compared to parental cells in vivo, but not in vitro. Additionally, no change in growth under hypoxic or starvation conditions was seen in these cells cultured two-dimensionally; however, HCT116-miR3148 cells maintained as three-dimensional spheroids were highly resistant to hypoxic conditions. HCT116-miR3148 cells were more sensitive to mitogen-activated protein kinase (MAPK) kinase inhibitors and extracellular signal-regulated kinase (ERK) inhibitors. Conclusion: MiR-3148 may be a novel onco-microRNA that protects cancer cells from serious stress conditions through the MAPK/ERK pathway, especially in vivo.
AB - Background/Aim: Alterations of microRNA expression in three-dimensional spheroids were examined to identify novel microRNAs that might be associated with tumorigenesis. Materials and Methods: Using microRNA microarray analysis, we screened for microRNAs that were dramatically up-regulated inside three-dimensional spheroids in genetically-modified HCT116 human colon cancer cells expressing Copepoda Green Fluorescent Protein under hypoxia. Results: miR-3148 was identified as a possible candidate onco-microRNA. A growth advantage of HCT116 cells stably expressing miR-3148 (HCT116-miR3148) was observed compared to parental cells in vivo, but not in vitro. Additionally, no change in growth under hypoxic or starvation conditions was seen in these cells cultured two-dimensionally; however, HCT116-miR3148 cells maintained as three-dimensional spheroids were highly resistant to hypoxic conditions. HCT116-miR3148 cells were more sensitive to mitogen-activated protein kinase (MAPK) kinase inhibitors and extracellular signal-regulated kinase (ERK) inhibitors. Conclusion: MiR-3148 may be a novel onco-microRNA that protects cancer cells from serious stress conditions through the MAPK/ERK pathway, especially in vivo.
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U2 - 10.21873/anticanres.12906
DO - 10.21873/anticanres.12906
M3 - Article
C2 - 30275189
AN - SCOPUS:85054067261
SN - 0250-7005
VL - 38
SP - 5693
EP - 5701
JO - Anticancer Research
JF - Anticancer Research
IS - 10
ER -
